• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Amplifluor 类 SNP 标记相对于 KASP 在植物基因型鉴定中的优势。

Advantages of Amplifluor-like SNP markers over KASP in plant genotyping.

机构信息

Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University, Astana, Kazakhstan.

School of Biological Sciences, Flinders University, Bedford Park, SA, Australia.

出版信息

BMC Plant Biol. 2017 Dec 28;17(Suppl 2):254. doi: 10.1186/s12870-017-1197-x.

DOI:10.1186/s12870-017-1197-x
PMID:29297326
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5751575/
Abstract

BACKGROUND

KASP (KBioscience Competitive Allele Specific PCR) and Amplifluor (Amplification with fluorescence) SNP markers are two prominent technologies based upon a shared identical Allele-specific PCR platform.

METHODS

Amplifluor-like SNP and KASP analysis was carried out using published and own design of Universal probes (UPs) and Gene-specific primers (GSPs).

RESULTS

Advantages of the Amplifluor-like system over KASP include the significantly lower costs and much greater flexibility in the adjustment and development of 'self-designed' dual fluorescently-labelled UPs and regular GSPs. The presented results include optimisation of 'tail' length in UPs and GSPs, protocol adjustment, and the use of various fluorophores in different qPCR instruments. The compatibility of the KASP Master-mix in both original and Amplifluor-like systems has been demonstrated in the presented results, proving their similar principles. Results of SNP scoring with rare alleles in addition to more frequently occurring alleles are shown.

CONCLUSIONS

The Amplifluor-like system produces SNP genotyping results with a level of sensitivity and accuracy comparable to KASP but at a significantly cheaper cost and with much greater flexibility for UPs with self-designed GSPs.

摘要

背景

KASP（KBioscience 竞争性等位基因特异性 PCR）和 Amplifluor（荧光扩增）SNP 标记物是两种基于相同等位基因特异性 PCR 平台的突出技术。

方法

使用已发表的和我们自己设计的通用探针 (UPs) 和基因特异性引物 (GSPs) 进行类似 Amplifluor 的 SNP 和 KASP 分析。

结果

与 KASP 相比，类似 Amplifluor 的系统具有显著更低的成本和更大的灵活性，可用于调整和开发“自行设计”的双荧光标记 UPs 和常规 GSPs。所呈现的结果包括 UP 和 GSP 中“尾巴”长度的优化、方案调整以及不同 qPCR 仪器中各种荧光团的使用。所呈现的结果证明了 KASP 主混合物在原始和类似 Amplifluor 系统中的兼容性，证明了它们具有相似的原理。除了更频繁出现的等位基因外，还展示了稀有等位基因的 SNP 评分结果。

结论

类似 Amplifluor 的系统产生 SNP 基因分型结果的灵敏度和准确性与 KASP 相当，但成本显著更低，并且具有更大的灵活性，可用于自行设计 GSP 的 UP。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d8e/5751575/cc7516a698b3/12870_2017_1197_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d8e/5751575/b3cf14b45656/12870_2017_1197_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d8e/5751575/8cf2c5a8bcc6/12870_2017_1197_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d8e/5751575/bc7e55e56b19/12870_2017_1197_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d8e/5751575/2b9fe7a3ef0c/12870_2017_1197_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d8e/5751575/e93baade960f/12870_2017_1197_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d8e/5751575/cc7516a698b3/12870_2017_1197_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d8e/5751575/b3cf14b45656/12870_2017_1197_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d8e/5751575/8cf2c5a8bcc6/12870_2017_1197_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d8e/5751575/bc7e55e56b19/12870_2017_1197_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d8e/5751575/2b9fe7a3ef0c/12870_2017_1197_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d8e/5751575/e93baade960f/12870_2017_1197_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d8e/5751575/cc7516a698b3/12870_2017_1197_Fig6_HTML.jpg

相似文献

1
Advantages of Amplifluor-like SNP markers over KASP in plant genotyping.Amplifluor 类 SNP 标记相对于 KASP 在植物基因型鉴定中的优势。
BMC Plant Biol. 2017 Dec 28;17(Suppl 2):254. doi: 10.1186/s12870-017-1197-x.
2
SNP Genotyping with Amplifluor-Like Method.SNP 基因分型与 Amplifluor 类似方法。
Methods Mol Biol. 2023;2638:201-219. doi: 10.1007/978-1-0716-3024-2_14.
3
Modified "Allele-Specific qPCR" Method for SNP Genotyping Based on FRET.基于荧光共振能量转移的改良“等位基因特异性定量PCR”法用于单核苷酸多态性基因分型
Front Plant Sci. 2022 Jan 10;12:747886. doi: 10.3389/fpls.2021.747886. eCollection 2021.
4
Amplifluor-Based SNP Genotyping.基于 Amplifluor 的 SNP 基因分型。
Methods Mol Biol. 2023;2638:191-200. doi: 10.1007/978-1-0716-3024-2_13.
5
SNP genotyping: the KASP assay.单核苷酸多态性基因分型：竞争性等位基因特异性PCR法
Methods Mol Biol. 2014;1145:75-86. doi: 10.1007/978-1-4939-0446-4_7.
6
Refinement of single-nucleotide polymorphism genotyping methods on human genomic DNA: amplifluor allele-specific polymerase chain reaction versus ligation detection reaction-TaqMan.人类基因组DNA上单核苷酸多态性基因分型方法的优化：荧光扩增等位基因特异性聚合酶链反应与连接检测反应- TaqMan技术比较
Anal Biochem. 2004 Jul 15;330(2):288-97. doi: 10.1016/j.ab.2004.03.035.
7
Comparison of TaqMan, KASP and rhAmp SNP genotyping platforms in hexaploid wheat.六倍体小麦中 TaqMan、KASP 和 rhAmp SNP 基因分型平台的比较。
PLoS One. 2019 May 22;14(5):e0217222. doi: 10.1371/journal.pone.0217222. eCollection 2019.
8
KASP: a high-throughput genotyping system and its applications in major crop plants for biotic and abiotic stress tolerance.KASP：一种高通量基因分型系统及其在主要作物中的生物和非生物胁迫耐受性方面的应用。
Mol Biol Rep. 2024 Apr 15;51(1):508. doi: 10.1007/s11033-024-09455-z.
9
Combining Single Nucleotide Polymorphism Genotyping Array with Bulked Segregant Analysis to Map a Gene Controlling Adult Plant Resistance to Stripe Rust in Wheat Line 03031-1-5 H62.结合单核苷酸多态性基因分型阵列与集群分离分析法定位小麦品系03031-1-5 H62中一个控制成株期抗条锈病的基因
Phytopathology. 2018 Jan;108(1):103-113. doi: 10.1094/PHYTO-04-17-0153-R. Epub 2017 Nov 9.
10
Universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR) for SNP genotyping.基于通用探针的中间引物触发 qPCR（UPIP-qPCR）用于 SNP 基因分型。
BMC Genomics. 2021 Nov 24;22(1):850. doi: 10.1186/s12864-021-08148-2.

引用本文的文献

1
An Assay for Variable Fragment Length Allele-Specific Genotyping.可变片段长度等位基因特异性基因分型分析
Methods Mol Biol. 2025;2943:1-17. doi: 10.1007/978-1-0716-4642-7_1.
2
Development and Validation of Kompetitive Allele-Specific Polymerase Chain Reaction Markers for Seed Protein Content in Soybean.大豆种子蛋白质含量竞争性等位基因特异性聚合酶链反应标记的开发与验证
Plants (Basel). 2024 Dec 13;13(24):3485. doi: 10.3390/plants13243485.
3
Evaluation of genetic diversity among olive trees ( L.) from Jordan.约旦油橄榄树（L.）遗传多样性评估

本文引用的文献

1
Genetic mapping and haplotype analysis of a locus for quantitative resistance to Fusarium graminearum in soybean accession PI 567516C.大豆种质资源 PI 567516C 中抗禾谷镰刀菌数量性状位点的遗传图谱构建和单倍型分析
Theor Appl Genet. 2017 May;130(5):999-1010. doi: 10.1007/s00122-017-2866-8. Epub 2017 Mar 8.
2
Single nucleotide polymorphism discrimination with and without an ethidium bromide intercalator.单核苷酸多态性的区分，有无溴化乙锭嵌入剂。
Anal Chim Acta. 2017 Feb 15;954:121-128. doi: 10.1016/j.aca.2016.12.011. Epub 2016 Dec 30.
3
Expression Level of the -Type Gene, Identified with Amplifluor SNP Markers, Correlates with Performance, and Tolerance to Dehydration in Bread Wheat Cultivars from Northern Kazakhstan.
Front Plant Sci. 2024 Aug 6;15:1437055. doi: 10.3389/fpls.2024.1437055. eCollection 2024.
4
Application of bulk segregant RNA-Seq (BSR-Seq) and allele-specific primers to study soybean powdery mildew resistance.应用 bulk segregant RNA-Seq（BSR-Seq）和等位基因特异性引物研究大豆白粉病抗性。
BMC Plant Biol. 2024 Mar 1;24(1):155. doi: 10.1186/s12870-024-04822-1.
5
Identification of qBK2.1, a novel QTL controlling rice resistance against Fusarium fujikuroi.新型数量性状基因座qBK2.1的鉴定，该基因座控制水稻对藤仓镰刀菌的抗性。
Bot Stud. 2023 Apr 20;64(1):11. doi: 10.1186/s40529-023-00375-y.
6
Chile Pepper () Breeding and Improvement in the "Multi-Omics" Era.“多组学”时代的辣椒育种与改良
Front Plant Sci. 2022 May 3;13:879182. doi: 10.3389/fpls.2022.879182. eCollection 2022.
7
Modified "Allele-Specific qPCR" Method for SNP Genotyping Based on FRET.基于荧光共振能量转移的改良“等位基因特异性定量PCR”法用于单核苷酸多态性基因分型
Front Plant Sci. 2022 Jan 10;12:747886. doi: 10.3389/fpls.2021.747886. eCollection 2021.
8
Quantitative Trait Loci Mapping and Development of KASP Marker Smut Screening Assay Using High-Density Genetic Map and Bulked Segregant RNA Sequencing in Sugarcane ( spp.).利用甘蔗（spp.）高密度遗传图谱和混合分离群体RNA测序进行数量性状位点定位及开发KASP标记黑穗病筛选分析方法
Front Plant Sci. 2022 Jan 5;12:796189. doi: 10.3389/fpls.2021.796189. eCollection 2021.
9
Expression of Specific Alleles of Zinc-Finger Transcription Factors, and , and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations.锌指转录因子的特定等位基因的表达，和 ，以及相应的 SNP 标记，与大麦群体的耐旱性有关。
Int J Mol Sci. 2021 Nov 10;22(22):12156. doi: 10.3390/ijms222212156.
10
and , Partner Genes of a Transcription Repressor, Coordinate Plant Development and Drought Tolerance in Spelt and Bread Wheat.并且，作为转录阻遏物的伴侣基因，在斯佩尔特小麦和普通小麦中协调植物发育和耐旱性。
Int J Mol Sci. 2020 Nov 5;21(21):8296. doi: 10.3390/ijms21218296.
用荧光定量SNP标记鉴定的γ型基因表达水平与哈萨克斯坦北部面包小麦品种的性能及脱水耐受性相关。
Front Plant Sci. 2016 Nov 18;7:1736. doi: 10.3389/fpls.2016.01736. eCollection 2016.
4
Identification of Genes Potentially Associated with the Fertility Instability of S-Type Cytoplasmic Male Sterility in Maize via Bulked Segregant RNA-Seq.通过混合分组RNA测序鉴定与玉米S型细胞质雄性不育育性不稳定性潜在相关的基因
PLoS One. 2016 Sep 26;11(9):e0163489. doi: 10.1371/journal.pone.0163489. eCollection 2016.
5
The eyespot resistance genes Pch1 and Pch2 of wheat are not homoeoloci.小麦的抗眼斑病基因Pch1和Pch2不是同源位点。
Theor Appl Genet. 2017 Jan;130(1):91-107. doi: 10.1007/s00122-016-2796-x. Epub 2016 Sep 24.
6
Genetic mapping of resistance to the Ug99 race group of Puccinia graminis f. sp. tritici in a spring wheat landrace CItr 4311.春小麦地方品种CItr 4311对小麦条锈菌Ug99小种的抗性遗传图谱构建
Theor Appl Genet. 2016 Nov;129(11):2161-2170. doi: 10.1007/s00122-016-2764-5. Epub 2016 Aug 20.
7
Validating DNA Polymorphisms Using KASP Assay in Prairie Cordgrass (Spartina pectinata Link) Populations in the U.S.利用竞争性等位基因特异性PCR（KASP）检测法验证美国草原网茅（Spartina pectinata Link）种群中的DNA多态性
Front Plant Sci. 2016 Jan 22;6:1271. doi: 10.3389/fpls.2015.01271. eCollection 2015.
8
Fine Mapping and Characterization of Candidate Genes that Control Resistance to Cercospora sojina K. Hara in Two Soybean Germplasm Accessions.两个大豆种质资源中控制对大豆尾孢菌抗性的候选基因的精细定位与特性分析
PLoS One. 2015 May 19;10(5):e0126753. doi: 10.1371/journal.pone.0126753. eCollection 2015.
9
SNP identification and marker assay development for high-throughput selection of soybean cyst nematode resistance.用于大豆胞囊线虫抗性高通量选择的单核苷酸多态性（SNP）鉴定及标记检测开发
BMC Genomics. 2015 Apr 18;16(1):314. doi: 10.1186/s12864-015-1531-3.
10
Specific PCR and real-time PCR assays for detection and quantitation of 'Candidatus Phytoplasma phoenicium'.用于检测和定量“嗜酸韧皮部杆菌（暂定种）”的特异性PCR和实时PCR检测方法
Mol Cell Probes. 2015 Feb;29(1):63-70. doi: 10.1016/j.mcp.2014.12.003. Epub 2014 Dec 25.