Kalendar Ruslan, Shevtsov Vladislav, Ismailova Aisulu
National Laboratory Astana, Nazarbayev University, Astana, Kazakhstan.
Department of Information Systems, S. Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan.
Methods Mol Biol. 2025;2943:1-17. doi: 10.1007/978-1-0716-4642-7_1.
Polymerase chain reaction (PCR) is a quick and easy technique used to detect nucleotide polymorphisms and sequence variations in various fields such as medicine, agriculture, and basic research. There exists a set of PCR variants, collectively called allele-specific PCR, which use competitive reactions in the presence of allele-specific primers to amplify only specific alleles. In this chapter, we present protocols for a bioinformatics tool that can be used to design PCR-based genotyping assays for variable fragment length allele-specific genotyping. This assay is designed in both directions and can target single-nucleotide polymorphisms and insertion/deletions. This chapter provides a step-by-step guide to design a multiplexed primer assay for variable fragment length allele-specific genotyping.
聚合酶链反应（PCR）是一种快速简便的技术，用于检测医学、农业和基础研究等各个领域中的核苷酸多态性和序列变异。存在一组PCR变体，统称为等位基因特异性PCR，它们在等位基因特异性引物存在下利用竞争性反应仅扩增特定等位基因。在本章中，我们介绍了一种生物信息学工具的方案，该工具可用于设计基于PCR的基因分型检测方法，用于可变片段长度等位基因特异性基因分型。该检测方法是双向设计的，可针对单核苷酸多态性和插入/缺失。本章提供了一个逐步指南，用于设计用于可变片段长度等位基因特异性基因分型的多重引物检测方法。
