* Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 369-873, Republic of Korea.
† Department of Food Science and Nutrition, Pusan National University, Busan 46241, Republic of Korea.
Am J Chin Med. 2018;46(1):157-174. doi: 10.1142/S0192415X1850009X. Epub 2018 Jan 3.
Cisplatin, a platinum chelate with potent antitumor activity against cancers of the testis, ovary, urinary bladder, prostate, and head and neck, has adverse effects on the kidney, bone marrow, and digestive organs, and its use is particularly limited by nephropathy as a side effect. In the present study, safflower seed extract was administered to a mouse model of cisplatin-induced acute renal failure to investigate its activity. Cisplatin (20[Formula: see text]mg/kg body weight) was administered by intraperitoneal injection to mice that had received oral safflower seed extract (100 or 200[Formula: see text]mg/kg body weight per day) for the preceding 2 days. Three days after the cisplatin injection, serum and renal biochemical factors; oxidative stress, inflammation, and apoptosis-related protein expression; and histological findings were evaluated. Cisplatin-treated control mice showed body-weight, food intake and water intake loss, and increased kidney weight, whereas the administration of safflower seed extract attenuated these effects ([Formula: see text], [Formula: see text]). Moreover, safflower seed extract significantly decreased the renal functional parameters urea nitrogen and creatinine in the serum ([Formula: see text] and [Formula: see text], respectively). Safflower seed extract also significantly reduced the enhanced levels of reactive oxygen species in the kidney observed following cisplatin treatment, with significance. The expression of proteins related to the anti-oxidant defense system in the kidney was down-regulated following cisplatin treatment, but safflower seed extract significantly up-regulated the expression of the anti-oxidant enzyme catalase. Furthermore, safflower seed extract reduced the overexpression of phosphor (p)-p38, nuclear factor-kappa B p65, cyclooxygenase-2, inducible nitric oxide synthase, ATR, p-p53, Bax, and caspase 3 proteins, and mice treated with safflower seed extract exhibited less renal histological damage. These results provide important evidence that safflower seed extract exerts a pleiotropic effect on several oxidative stress- and apoptosis-related parameters and has a renoprotective effect in cisplatin-treated mice.
红花籽油提取物对顺铂诱导的急性肾衰竭小鼠模型的作用研究。将顺铂（20[Formula: see text]mg/kg 体重）腹膜内注射到先前连续 2 天接受红花籽油提取物（100 或 200[Formula: see text]mg/kg 体重/天）口服处理的小鼠中。顺铂注射后 3 天，评估血清和肾脏生化因子；氧化应激、炎症和细胞凋亡相关蛋白表达；以及组织学发现。顺铂处理的对照组小鼠表现出体重、食物和水摄入减少以及肾脏重量增加，而红花籽油提取物的给药减轻了这些作用（[Formula: see text]，[Formula: see text]）。此外，红花籽油提取物显著降低了血清中肾功参数尿素氮和肌酐（[Formula: see text]和[Formula: see text]，分别）。红花籽油提取物还显著降低了顺铂处理后观察到的肾脏中活性氧水平的增强，具有统计学意义。顺铂处理后肾脏中抗氧化防御系统相关蛋白的表达下调，但红花籽油提取物显著上调了抗氧化酶过氧化氢酶的表达。此外，红花籽油提取物减少了磷酸化（p）-p38、核因子-κB p65、环氧化酶-2、诱导型一氧化氮合酶、ATR、p-p53、Bax 和 caspase 3 蛋白的过度表达，并且用红花籽油提取物处理的小鼠表现出较少的肾脏组织学损伤。这些结果提供了重要证据，表明红花籽油提取物对几种氧化应激和细胞凋亡相关参数具有多效作用，并对顺铂处理的小鼠具有肾脏保护作用。
