Dhanapal Jeevitha, Balaraman Ravindrran Malathy
a Faculty of Science and Humanities (Life Sciences), Department of Biotechnology , Sathyabama Institute of Science and Technology , Chennai , India.
b Department of Microbiology , Sathyabama University Dental College and Hospital , Chennai , India.
Artif Cells Nanomed Biotechnol. 2018;46(sup1):274-282. doi: 10.1080/21691401.2017.1422130. Epub 2018 Jan 3.
The aim of the study was to synthesize nanoparticles (NPs) with chitosan (CS), and poly (lactic acid) (PLA) as a carrier for the drug piceatannol (PIC). The synthesized nanoparticles form the composite of polymeric-drug nanoparticles (CS/PLA-PIC NPs) by dropping method. The preliminary and stability studies were determined for the polymers drug-loading capacity and encapsulation efficiencies. The in vitro drug release study showed that NPs provided a continuous release of the entrapped PIC. The NPs found to be a good scavenger for DPPH, SOD and NO radicals. MTT and LDH assays revealed higher cytotoxic efficacy of CS/PLA-PIC NPs in HepG2, A549 and MCF7 cells compared to CS-PLA NPs and PIC. Dual staining results showed the early/late-stages of apoptotic and necrotic cells. Furthermore, cells treated with CS/PLA-PIC NPs showed fragmenting DNA and also demonstrated for apoptotic cells percentage by flow cytometry. These results suggested that upon CS/PLA-PIC NPs exposure leads to decrease in cancer cell viability due to apoptosis.
本研究的目的是合成以壳聚糖（CS）和聚乳酸（PLA）为药物白皮杉醇（PIC）载体的纳米颗粒（NPs）。通过滴加法合成的纳米颗粒形成了聚合物-药物纳米颗粒（CS/PLA-PIC NPs）复合物。对聚合物的载药量和包封率进行了初步和稳定性研究。体外药物释放研究表明，纳米颗粒能够持续释放包封的PIC。发现纳米颗粒对DPPH、超氧化物歧化酶（SOD）和一氧化氮（NO）自由基具有良好的清除作用。MTT和乳酸脱氢酶（LDH）检测显示，与CS-PLA纳米颗粒和PIC相比，CS/PLA-PIC纳米颗粒对HepG2、A549和MCF7细胞具有更高的细胞毒性作用。双重染色结果显示了凋亡和坏死细胞的早期/晚期阶段。此外，用CS/PLA-PIC纳米颗粒处理的细胞显示出DNA片段化，并且通过流式细胞术证实了凋亡细胞的百分比。这些结果表明，暴露于CS/PLA-PIC纳米颗粒会导致癌细胞活力因凋亡而降低。
