Ding Hao, Wang Yafeng, Chen Yuanmei, Liu Chao, Qiu Hao, Kang Mingqiang, Tang Weifeng
Department of Respiratory Disease, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China.
Department of Cardiology, The People's Hospital of Xishuangbanna Dai Autonomous Prefecture, Jinghong, Yunnan Province, China.
Oncotarget. 2017 Dec 4;8(66):110326-110336. doi: 10.18632/oncotarget.22887. eCollection 2017 Dec 15.
Previous reports implicated 5,10- () polymorphisms acted as a potential risk factor for several cancers. In order to explore the effect of SNPs on non-small cell lung cancer (NSCLC), we selected tagging single nucleotide polymorphisms (SNPs) and carried out a case-control study to determine the potential relationship of SNPs with NSCLC risk. Our study consisted of 521 NSCLC patients and 1,030 non-cancer controls. SNPs were genotyped by SNPscan genotyping assay. Using four genetic models (additive, Homozygote, dominant, recessive), the genotype frequencies were compared using the chi-squared () test. Crude/adjusted odds ratios (ORs) with their 95% confidence intervals (CIs) were used to assess the difference for the genotype distribution. We found that MTHFR rs1801133 G>A polymorphism decreased the risk of overall NSCLC. In a subgroup analysis, rs1801133 G>A polymorphism also decreased NSCLC risk in female, < 60 years and never smoking subgroups. However, we identified that MTHFR rs4845882 G>A polymorphism was associated with the development of NSCLC in female subgroup. In addition, rs9651118 T>C polymorphism increased the risk of NSCLC in < 60 years, never smoking and BMI < 24 kg/m subgroups. In conclusion, the current study highlights rs1801133 G>A variants decreases the risk of NSCLC. Nevertheless, MTHFR rs4845882 G>A and rs9651118 T > C polymorphisms may be associated with NSCLC susceptibility. Well-designed large-scale studies are needed to confirm these findings and explore the interactions of gene-gene and gene-environment involved in SNPs and NSCLC.
先前的报告表明，5,10-（）多态性是几种癌症的潜在危险因素。为了探讨单核苷酸多态性（SNPs）对非小细胞肺癌（NSCLC）的影响，我们选择了标签单核苷酸多态性，并进行了一项病例对照研究，以确定SNPs与NSCLC风险之间的潜在关系。我们的研究包括521例NSCLC患者和1030例非癌症对照。通过SNPscan基因分型检测对SNPs进行基因分型。使用四种遗传模型（加性、纯合子、显性、隐性），采用卡方（）检验比较基因型频率。使用粗/调整比值比（ORs）及其95%置信区间（CIs）来评估基因型分布的差异。我们发现，亚甲基四氢叶酸还原酶（MTHFR）rs1801133 G>A多态性降低了总体NSCLC的风险。在亚组分析中，rs1801133 G>A多态性在女性、<60岁和从不吸烟亚组中也降低了NSCLC风险。然而，我们发现MTHFR rs4845882 G>A多态性与女性亚组中NSCLC的发生有关。此外，rs9651118 T>C多态性在<60岁、从不吸烟和体重指数（BMI）<24 kg/m亚组中增加了NSCLC风险。总之，当前研究强调rs1801133 G>A变异降低了NSCLC风险。然而，MTHFR rs4845882 G>A和rs9651118 T>C多态性可能与NSCLC易感性有关。需要设计良好的大规模研究来证实这些发现，并探索涉及SNPs和NSCLC的基因-基因和基因-环境相互作用。
