Zhou Weiguang, Xiao Yingxuan, Jiang Yifan, Zou Aoxiang, Ruan Jiangyi, Feng Xianhong, Li Jing, Chen Bifeng
Department of Biological Science and Technology, School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, Hubei, 430070, China.
Institute of WUT-AMU, Wuhan University of Technology, Wuhan, Hubei, 430070, China.
Biochem Genet. 2024 Sep 21. doi: 10.1007/s10528-024-10929-9.
Recently, rs9651118 in the MTHFR gene and rs2790 in the TYMS gene have been repeatedly studied for their contribution to cancer risk. However, the results remain conflicting rather than conclusive. Therefore, we here conducted a replication case-control study and a meta-analysis to comprehensively examine the contribution of rs9651118 and rs2790 to cancer risk. A total of 1727 patients with colorectal/gastric/liver (787/460/480) cancer and 800 healthy controls were recruited, and the Sanger sequencing was applied to genotype rs9651118 and rs2790. Besides, a total of 23 eligible studies were included in the following meta-analysis. After Bonferroni correction, the results of case-control study suggested that significant associations between rs9651118 and colorectal cancer (CRC) risk, rs9651118 and gastric cancer (GC) risk, and rs2790 and liver cancer (LC) risk were identified in Hubei Chinese population. The results of meta-analysis indicated that after Bonferroni correction, both rs9651118 and rs2790 were significantly associated with total cancer risk especially in Asian population and based on Sanger sequencing method, rs9651118 was significantly associated with breast cancer (BC) risk, and rs2790 was significantly associated with the risk of CRC and GC. In conclusion, the present findings revealed that the MTHFR gene rs9651118 may participate in the risk of total cancer (especially BC) in Asian population, and the TYMS gene rs2790 may be associated with the risk of total cancer (especially CRC) in Asian population and also the risk of GC in total population.
最近，MTHFR基因中的rs9651118和TYMS基因中的rs2790对癌症风险的影响已被反复研究。然而，结果仍然相互矛盾，而非确凿无疑。因此，我们在此开展了一项重复病例对照研究和一项荟萃分析，以全面检验rs9651118和rs2790对癌症风险的影响。共招募了1727例结直肠癌/胃癌/肝癌患者（787/460/480例）和800名健康对照者，并采用桑格测序法对rs9651118和rs2790进行基因分型。此外，以下荟萃分析共纳入了23项符合条件的研究。经Bonferroni校正后，病例对照研究结果表明，在湖北汉族人群中，rs9651118与结直肠癌（CRC）风险、rs9651118与胃癌（GC）风险以及rs2790与肝癌（LC）风险之间存在显著关联。荟萃分析结果表明，经Bonferroni校正后，rs9651118和rs2790均与总体癌症风险显著相关，尤其是在亚洲人群中；基于桑格测序法，rs9651118与乳腺癌（BC）风险显著相关，rs2790与CRC和GC风险显著相关。总之，本研究结果表明，MTHFR基因rs9651118可能参与亚洲人群总体癌症（尤其是BC）的风险，而TYMS基因rs2790可能与亚洲人群总体癌症（尤其是CRC）风险以及总体人群的GC风险相关。
