Lainson R, Shaw J J
Wellcome Parasitology Unit, Pará, Brazil.
Ann Parasitol Hum Comp. 1989;64(1):3-9. doi: 10.1051/parasite/19896413.
A new leishmanial parasite, Leishmania (Viannia) naiffi sp. n., is described from the nine-banded armadillo, Dasypus novemcinctus (Edentata: Dasypodidae), from Para State, north Brazil. The parasite grows luxuriantly in Diffco blood-agar medium (B47), but poorly in the skin of intradermally inoculated hamsters. A comparison of isoenzyme profiles by starch gel electrophoresis separates the parasite from L. (V) braziliensis and L. (V.) guyanensis by the enzymes ASAT, ALAT, PGM, GPI, G6PD, PEP, MPI and GD, and from Leishmania (Leishmania) chagasi, L. (L.) amazonensis and L. (L.) deanei by ASAT, ALAT, PGM, GPI, MPI, G6PD, MDH, PEP and ACON. Finally, L. (V.) naiffi is serologically differentiated from L. (V.) braziliensis, L. (V.) guyanensis and L. (V.) panamensis on monoclonal antibodies specific for these parasites.
一种新的利什曼原虫寄生虫,即纳菲利什曼原虫(维安尼亚亚属)新种,是从巴西北部帕拉州的九带犰狳(Dasypus novemcinctus,贫齿目:犰狳科)中发现并描述的。该寄生虫在Diffco血琼脂培养基(B47)中生长旺盛,但在皮内接种仓鼠的皮肤中生长不佳。通过淀粉凝胶电泳对同工酶谱进行比较,该寄生虫在ASAT、ALAT、PGM、GPI、G6PD、PEP、MPI和GD酶方面与巴西利什曼原虫(维安尼亚亚属)和圭亚那利什曼原虫(维安尼亚亚属)不同,在ASAT、ALAT、PGM、GPI、MPI、G6PD、MDH、PEP和ACON酶方面与恰加斯利什曼原虫(利什曼原虫亚属)、亚马逊利什曼原虫(利什曼原虫亚属)和德内利什曼原虫(利什曼原虫亚属)不同。最后,利用针对这些寄生虫的单克隆抗体,纳菲利什曼原虫(维安尼亚亚属)在血清学上与巴西利什曼原虫(维安尼亚亚属)、圭亚那利什曼原虫(维安尼亚亚属)和巴拿马利什曼原虫(维安尼亚亚属)得以区分。