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巴西的利什曼病:十七。来自帕拉州九带犰狳(贫齿目)的一种利什曼原虫的酶学特性

Leishmaniasis in Brazil: XVII. Enzymic characterization of a Leishmania from the armadillo, Dasypus novemcinctus (Edentata), from Pará State.

作者信息

Lainson R, Shaw J J, Miles M A, Póvoa M

出版信息

Trans R Soc Trop Med Hyg. 1982;76(6):810-11. doi: 10.1016/0035-9203(82)90115-8.

Abstract

A comparison of enzyme profiles, by starch-gel electrophoresis, has distinguished a Leishmania of armadillos (Dasypus novemcinctus), from Pará State, north Brazil, from Leishmania braziliensis braziliensis, L. braziliensis guyanensis, L. mexicana amazonensis, L. donovani sensu lato (from Bahia, Brazil), and L. hertigi deanei. The parasite was separated from L. b. braziliensis and L. b. guyanensis by 8 of the 14 enzymes used (ASAT, ALAT, PGM, GPI, G6PD, PEP, MPI and GD), although differences in the mobility of some of the enzymes were small. At least 9 of the enzymes separated the organism from L. m. amazonensis, L. donovani s.l., and L. h. deanei.

摘要

通过淀粉凝胶电泳对酶谱进行比较,已将来自巴西北部帕拉州的犰狳(九带犰狳)体内的利什曼原虫与巴西利什曼原虫指名亚种、圭亚那利什曼原虫、亚马逊利什曼原虫、杜氏利什曼原虫复合种(来自巴西巴伊亚州)以及赫氏利什曼原虫区分开来。在所使用的14种酶中,有8种酶可将该寄生虫与巴西利什曼原虫指名亚种和圭亚那利什曼原虫区分开(天冬氨酸转氨酶、丙氨酸转氨酶、磷酸葡萄糖变位酶、葡萄糖磷酸异构酶、葡萄糖-6-磷酸脱氢酶、磷酸烯醇丙酮酸羧激酶、甘露糖磷酸异构酶和葡萄糖脱氢酶),不过部分酶的迁移率差异较小。至少9种酶可将该生物体与亚马逊利什曼原虫、杜氏利什曼原虫复合种以及赫氏利什曼原虫区分开。

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