[δ12-前列腺素J2对G1期累积细胞中蛋白质和RNA含量的影响]
[Changes of protein and RNA content in accumulated G1 cells by delta12-PGJ2].
作者信息
Ishikawa H, Tagawa Y, Sato T, Nakao H, Hara S, Shiraishi E, Kawabuchi T, Oh S, Shimoyama T, Tomita M
机构信息
First Dept. of Surgery, Nagasaki University School of Medicine.
出版信息
Gan To Kagaku Ryoho. 1989 Mar;16(3 Pt 1):405-10.
Protein and RNA content in accumulated G1 cells was measured with B16 melanoma cells by flow cytometer to study the mechanism of antineoplastic action of delta 12PGJ2. DNA histogram showed that, after a 6 hr exposure to delta 12PGJ2, the percentage of tumor cells in G2M phase increased, and G1 accumulation with depletion of cell population in S phase was seen after 12-24 hr exposure to 2.0 micrograms/ml of delta 12 PGJ2. Protein content in accumulated G1 cells increased at 6 hr after exposure, and subsequently decreased. However, the protein content treated with delta 12PGJ2 was higher than that of the control. A reduction of RNA content in G1 cells was seen after delta 12PGJ2 exposure. As the result, it was found that delta 12PGJ2 made cell progression ceased in G1 phase of cell cycle, and where protein and RNA content was higher than that of control. It is suggested that some hyper-metabolite state was caused by delta 12 PGJ2 in cultured B16 melanoma cells.
用流式细胞仪检测B16黑色素瘤细胞中累积的G1期细胞的蛋白质和RNA含量，以研究δ12 -前列腺素J2（delta 12PGJ2）的抗肿瘤作用机制。DNA直方图显示，在暴露于δ12PGJ2 6小时后，处于G2M期的肿瘤细胞百分比增加，在暴露于2.0微克/毫升的δ12 -前列腺素J2 12 - 24小时后，可见G1期累积且S期细胞群体减少。累积的G1期细胞中的蛋白质含量在暴露后6小时增加，随后下降。然而，用δ12PGJ2处理的蛋白质含量高于对照组。在暴露于δ12PGJ2后，可见G1期细胞中的RNA含量降低。结果发现，δ12PGJ2使细胞周期在G1期停止，且此时蛋白质和RNA含量高于对照组。提示δ12 -前列腺素J2在培养的B16黑色素瘤细胞中引起了某种高代谢状态。
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