PUCRS, Escola de Ciências, Laboratório de Imunologia e Microbiologia, Porto Alegre, RS, Brazil.
PUCRS, Escola de Ciências, Programa de Pós-graduação em Zoologia, Porto Alegre, RS, Brazil.
Future Microbiol. 2018 Feb;13:209-219. doi: 10.2217/fmb-2017-0153. Epub 2018 Jan 5.
To evaluate the influence of aeration on persister levels from Acinetobacter calcoaceticus-baumannii isolates exposed to meropenem or tobramycin, as well as analyze morphological and structural changes in persisters.
MATERIALS & METHODS: Levels of persisters were determined after a 48-h exposure to tobramycin or meropenem under aerated or static conditions, and persisters were analyzed by scanning and transmission electron microscopy.
The fractions of persisters varied between isolates. Aeration reduced cell survival under each antibiotic treatment, and cell survival decreased as the tobramycin concentration was increased. Interestingly, division septa were observed in persisters by electron microscopy.
Aeration may have stimulated bacterial growth, providing more targets for antibiotic action and leading to increased production of reactive oxygen species, which decreased levels of persisters.
评估在暴露于美罗培南或妥布霉素的情况下，需氧对鲍曼不动杆菌分离株的持续生存者水平的影响，并分析持续生存者的形态和结构变化。
在需氧或静态条件下暴露于妥布霉素或美罗培南 48 小时后，测定持续生存者的水平，并通过扫描和透射电子显微镜分析持续生存者。
持续生存者的比例在不同分离株之间有所差异。需氧条件下，每种抗生素处理均降低了细胞存活率，且随着妥布霉素浓度的增加，细胞存活率降低。有趣的是，电子显微镜观察到了持续生存者中的分裂隔膜。
需氧可能刺激了细菌的生长，为抗生素作用提供了更多的靶点，并导致活性氧的产生增加，从而降低了持续生存者的水平。
