美罗培南对鲍曼不动杆菌持留菌细胞的影响。
Effects of meropenem exposure in persister cells of Acinetobacter calcoaceticus-baumannii.
作者信息
Gallo Stephanie Wagner, Donamore Bruna Kern, Pagnussatti Vany Elisa, Ferreira Carlos Alexandre Sanchez, de Oliveira Sílvia Dias
机构信息
Laboratório de Imunologia e Microbiologia, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil.
Departamento de Microbiologia, Laboratório de Patologia Clínica, Hospital São Lucas, Pontifícia Universidade Católica do Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil.
出版信息
Future Microbiol. 2017 Feb;12:131-140. doi: 10.2217/fmb-2016-0118. Epub 2017 Jan 23.
AIM
To evaluate the influence of meropenem in the Acinetobacter calcoaceticus-baumannii (ACB) persister levels.
METHODS
Persister levels in planktonic and biofilm cultures from ACB isolates were evaluated after exposure to different meropenem concentrations.
RESULTS
A high variability of persister fractions was observed among the isolates cultured under planktonic and biofilm conditions. Meropenem concentration did not influence persister fractions, even when far above the MIC. No correlation was found between persister levels and biofilm biomass.
CONCLUSION
The magnitude of persister levels from ACB planktonic and, particularly, biofilm cultures exposed to meropenem was independent of the antibiotic concentration, dosing regimen and biofilm biomass. These findings, in a context of meropenem failure to treat chronic infections, strengthen the importance of understanding persister behavior.
目的
评估美罗培南对醋酸钙不动杆菌-鲍曼不动杆菌（ACB）持留菌水平的影响。
方法
将ACB分离株暴露于不同浓度的美罗培南后，评估其浮游菌和生物被膜培养物中的持留菌水平。
结果
在浮游菌和生物被膜条件下培养的分离株中，观察到持留菌比例存在高度变异性。即使美罗培南浓度远高于最低抑菌浓度（MIC），其浓度也不会影响持留菌比例。未发现持留菌水平与生物被膜生物量之间存在相关性。
结论
暴露于美罗培南的ACB浮游菌尤其是生物被膜培养物中的持留菌水平高低，与抗生素浓度、给药方案和生物被膜生物量无关。在美罗培南治疗慢性感染失败的情况下，这些发现强化了了解持留菌行为的重要性。
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