The Medical Laboratory Diagnosis Center, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong, P.R. China.
The Department of Gynecology, People's Hospital of Rizhao, Rizhao, Shandong, P.R. China.
Histol Histopathol. 2018 Jul;33(7):673-680. doi: 10.14670/HH-11-960. Epub 2018 Jan 5.
Cathepsin K, or CTSK, has been found to be involved in the peritoneal metastasis of ovarian carcinoma. However, the expression and clinicopathological significance of CTSK remains unknown in epithelial ovarian cancer (EOC). The aim of the present study was to investigate the expression of CTSK and its clinicopathological significance in EOC. CTSK expression was evaluated using immunohistochemistry in EOC tissue microarray. The expression of CTSK in EOC was displayed to be markedly higher than that of adjacent normal control. In addition, CSTK expression was shown to be remarkably associated with metastases and inferior overall prognosis of EOC. In vitro, Knock-down of CTSD was exhibited to be able to suppress migration and invasion in EOC cell lines OV-2008 but not proliferation in OV-2008. Together, our data showed that elevated CTSD in EOC can potentiate the metastasis of EOC cells, suggesting that targeting CTSD might be used as a novel therapeutic target for EOC.
组织蛋白酶 K（Cathepsin K，CTSK）已被发现参与卵巢癌的腹膜转移。然而，在卵巢上皮性癌（epithelial ovarian cancer，EOC）中，CTSK 的表达及其与临床病理特征的关系尚不清楚。本研究旨在探讨 CTSK 在 EOC 中的表达及其临床病理意义。采用免疫组织化学方法对 EOC 组织微阵列进行 CTSK 表达检测。结果显示，CTSK 在 EOC 中的表达明显高于相邻正常对照。此外，CTSK 的表达与 EOC 的转移和整体预后不良显著相关。在体外，CTSK 的敲低能够抑制 EOC 细胞系 OV-2008 的迁移和侵袭，但对 OV-2008 的增殖没有影响。综上所述，EOC 中 CTSD 的升高可增强 EOC 细胞的转移能力，提示靶向 CTSD 可能作为 EOC 的一种新的治疗靶点。
