Wages D P, Ficken M D
Department of Food Animal and Equine Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Avian Dis. 1989 Jan-Mar;33(1):191-4.
Cryptosporidiosis and turkey viral hepatitis were diagnosed in 25-day-old turkeys with increased mortality, enteritis, and stunting. Necropsy lesions included foul-smelling, tan-colored, loose feces in the terminal small intestines and ceca. The small intestines and ceca were congested. Multiple white to gray foci were present in the liver and pancreas. Mortality attributed to these conditions was estimated at 4.9%. Microscopic lesions included necrosis with accumulations of macrophages in the liver and pancreas. Bile-duct hyperplasia was present in the livers. Microscopic intestinal lesions consisted of mild to moderate atrophy of the villi of the distal small intestine (ileo-cecal junction) with infiltration of the lamina propria by mononuclear cells. Cryptosporidia were identified and appeared to be attached to villous epithelial cells of the terminal small intestines. Cryptosporidium sp. oocysts were also confirmed by fluorescent microscopic examination of feces stained auramine O. Reovirus was isolated on chicken embryo liver cells from affected livers.
在25日龄火鸡中诊断出隐孢子虫病和火鸡病毒性肝炎，这些火鸡出现死亡率增加、肠炎和发育迟缓症状。尸检病变包括末端小肠和盲肠中有恶臭味、黄褐色、稀软的粪便。小肠和盲肠充血。肝脏和胰腺出现多个白色至灰色病灶。由这些病症导致的死亡率估计为4.9%。微观病变包括肝脏和胰腺中的坏死以及巨噬细胞聚集。肝脏出现胆管增生。微观肠道病变包括远端小肠（回盲交界处）绒毛轻度至中度萎缩，固有层有单核细胞浸润。鉴定出隐孢子虫，其似乎附着在末端小肠的绒毛上皮细胞上。通过对用金胺O染色的粪便进行荧光显微镜检查，也证实了隐孢子虫卵囊。从受影响的肝脏中，在鸡胚肝细胞上分离出呼肠孤病毒。
