Fujiwara H, Tanaami S, Yamaguchi M, Yoshino T
Natl Inst Anim Health Q (Tokyo). 1975 Summer;15(2):68-75.
In the summer of 1972, a disease of turkeys manifesting hemorrhagic diarrhea as a main clinical symptom and hemorrhagic enteritis as a necropsy finding broke out on a turkey farm in Japan. Seven-week-old Large White turkeys suffering from this disease were studied histopathologically and electron microscopically. Clinically, affected birds showed bloody diarrhea. Death occurred to them after an acute course. In the blood film, immature monocytes were higher in count in them than in healthy birds. Necropsy revealed a number of dark red bloody clots in the intestinal tract, many petechiae in the mucous membrane of small intestine and ceca, and atrophy of the spleen. The histopathological changes characteristic of this disease were acute hemorrhagic enteritis, degenerative changes of lymphatic tissue, proliferation of reticuloendothelial cells all over the body, and formation of intranuclear inclusion bodies in these cells. By electron microscopy, viral particles showing a crystalline array were found in the nuclei of reticuloendothelial cells. Viral particles which had electron-dense nucleoids and a naked hexagonal shape were about 80 nm in average diameter. The ultrastructural features of those inclusion bodies were identical with those of avian adenovirus.
1972年夏天，日本一家火鸡养殖场爆发了一种以出血性腹泻为主要临床症状、出血性肠炎为尸检结果的火鸡疾病。对患有这种疾病的7周龄大白火鸡进行了组织病理学和电子显微镜研究。临床上，患病火鸡表现为血性腹泻。病程急性，随后死亡。血液涂片显示，患病火鸡的未成熟单核细胞数量高于健康火鸡。尸检发现肠道内有许多暗红色血凝块，小肠和盲肠黏膜有许多瘀点，脾脏萎缩。这种疾病的组织病理学变化特征为急性出血性肠炎、淋巴组织变性、全身网状内皮细胞增生以及这些细胞内形成核内包涵体。通过电子显微镜观察，在网状内皮细胞的细胞核中发现了呈晶体排列的病毒颗粒。具有电子致密核仁且呈无包膜六边形的病毒颗粒平均直径约为80纳米。这些包涵体的超微结构特征与禽腺病毒的特征相同。
