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用于 3D 生物打印的海藻酸盐-明胶水凝胶的机械性能。

Mechanical behaviour of alginate-gelatin hydrogels for 3D bioprinting.

机构信息

Vascular Engineering Laboratory, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands and Centre for Medical Research, The University of Western Australia, Perth, Australia; School of Mechanical and Chemical Engineering, The University of Western Australia, Perth, Australia.

Ear Science Institute Australia, Perth, Australia.

出版信息

J Mech Behav Biomed Mater. 2018 Mar;79:150-157. doi: 10.1016/j.jmbbm.2017.12.018. Epub 2017 Dec 21.

Abstract

Hydrogels comprised of alginate and gelatin have demonstrated potential as biomaterials in three dimensional (3D) bioprinting applications. However, as with all hydrogel-based biomaterials used in extrusion-based bioprinting, many parameters influence their performance and there is limited data characterising the behaviour of alginate-gelatin (Alg-Gel) hydrogels. Here we investigated nine Alg-Gel blends by varying the individual constituent concentrations. We tested samples for printability and print accuracy, compressive behaviour and change over time, and viability of encapsulated mesenchymal stem cells in bioprinted constructs. Printability tests showed a decrease in strand width with increasing concentrations of Alg-Gel. However due to the increased viscosity associated with the higher Alg-Gel concentrations, the minimum width was found to be 0.32mm before blends became too viscous to print. Similarly, printing accuracy was increased in higher concentrations, exceeding 90% in some blends. Mechanical properties were assessed through uniaxial compression testing and it was found that increasing concentrations of both Alg and Gel resulted in higher compressive modulus. We also deemed 15min crosslinking in calcium chloride to be sufficient. From our data, we propose a blend of 7%Alg-8%Gel that yields high printability, mechanical strength and stiffness, and cell viability. However, we found the compressive behaviour of Alg-Gel to reduce rapidly over time and especially when incubated at 37°C. Here we have reported relevant data on Alg-Gel hydrogels for bioprinting. We tested for biomaterial properties and show that these hydrogels have many desirable characteristics that are highly tunable. Though further work is needed before practical use in vivo can be achieved.

摘要

水凝胶由藻酸盐和明胶组成,已被证明具有作为三维(3D)生物打印应用的生物材料的潜力。然而,与所有用于挤出式生物打印的基于水凝胶的生物材料一样,许多参数会影响其性能,并且用于描述藻酸盐-明胶(Alg-Gel)水凝胶行为的数据有限。在这里,我们通过改变单个成分的浓度来研究了 9 种 Alg-Gel 混合物。我们测试了样品的可打印性和打印精度、压缩性能以及随时间的变化,以及生物打印结构中包封的间充质干细胞的活力。打印性测试表明,随着 Alg-Gel 浓度的增加,丝宽度减小。然而,由于Alg-Gel 浓度较高会导致增加的粘度,因此发现混合物的最小宽度为 0.32mm,然后混合物变得过于粘稠而无法打印。同样,在较高浓度下打印精度增加,在某些混合物中超过 90%。通过单轴压缩测试评估了机械性能,发现Alg 和 Gel 的浓度增加会导致较高的压缩模量。我们还认为氯化钙中 15min 的交联时间是足够的。根据我们的数据,我们提出了一种 7%Alg-8%Gel 的混合物,该混合物具有较高的可打印性、机械强度和刚性以及细胞活力。然而,我们发现 Alg-Gel 的压缩性能随时间迅速降低,尤其是在 37°C 下孵育时。在这里,我们报告了用于生物打印的 Alg-Gel 水凝胶的相关数据。我们测试了生物材料的特性,并表明这些水凝胶具有许多高度可调的理想特性。尽管在实际体内应用之前还需要进一步的工作。

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