Vascular Engineering Laboratory, Harry Perkins Institute of Medical Research, Nedlands, and Centre for Medical Research, The University of Western Australia, Crawley, Perth, Australia; School of Mechanical and Chemical Engineering, The University of Western Australia, Perth, Australia.
School of Mechanical and Chemical Engineering, The University of Western Australia, Perth, Australia.
J Mech Behav Biomed Mater. 2018 Jan;77:389-399. doi: 10.1016/j.jmbbm.2017.09.031. Epub 2017 Sep 28.
Hydrogels containing hyaluronic acid (HA) and methylcellulose (MC) have shown promising results for three dimensional (3D) bioprinting applications. However, several parameters influence the applicability bioprinting and there is scarce data in the literature characterising HAMC. We assessed eight concentrations of HAMC for printability, swelling and stability over time, rheological and structural behaviour, and viability of mesenchymal stem cells. We show that HAMC blends behave as viscous solutions at 4°C and have faster gelation times at higher temperatures, typically gelling upon reaching 37°C. We found the storage, loss and compressive moduli to be dependent on HAMC concentration and incubation time at 37°C, and show the compressive modulus to be strain-rate dependent. Swelling and stability was influenced by time, more so than pH environment. We demonstrated that mesenchymal stem cell viability was above 75% in bioprinted structures and cells remain viable for at least one week after 3D bioprinting. The mechanical properties of HAMC are highly tuneable and we show that higher concentrations of HAMC are particularly suited to cell-encapsulated 3D bioprinting applications that require scaffold structure and delivery of cells.
含透明质酸 (HA) 和甲基纤维素 (MC) 的水凝胶在三维 (3D) 生物打印应用中显示出有前景的结果。然而，有几个参数会影响生物打印的适用性，并且文献中关于 HAMC 的数据很少。我们评估了 HAMC 的八种浓度在可打印性、溶胀和随时间的稳定性、流变学和结构行为以及间充质干细胞的活力方面的表现。我们表明，HAMC 混合物在 4°C 下表现为粘性溶液，在较高温度下具有更快的胶凝时间，通常在达到 37°C 时胶凝。我们发现存储、损耗和压缩模量取决于 HAMC 浓度和在 37°C 下的孵育时间，并且显示压缩模量是应变率依赖性的。溶胀和稳定性受时间影响较大，而受 pH 环境影响较小。我们证明了在生物打印结构中，间充质干细胞的活力高于 75%，并且在 3D 生物打印后至少一周内细胞仍然存活。HAMC 的机械性能具有高度可调性，我们表明较高浓度的 HAMC 特别适合需要支架结构和细胞输送的细胞包封 3D 生物打印应用。
