来自牛突触质膜的树突毒素受体。某些电压激活钾通道假定成分的结合特性、纯化及亚基组成。
Dendrotoxin acceptor from bovine synaptic plasma membranes. Binding properties, purification and subunit composition of a putative constituent of certain voltage-activated K+ channels.
作者信息
Parcej D N, Dolly J O
机构信息
Department of Biochemistry, Imperial College, London, U.K.
出版信息
Biochem J. 1989 Feb 1;257(3):899-903. doi: 10.1042/bj2570899.
Abstract
Dendrotoxin is a snake polypeptide that blocks selectively and potently certain voltage-sensitive, fast-activating K+ channels in the nervous system, where it binds with high affinity to membranous acceptors. Herein, the acceptor protein for dendrotoxin in bovine synaptic membranes is solubilized in active form and its complete purification achieved by affinity chromatography, involving a novel elution procedure. This putative K+-channel constituent is shown to be a large oligomeric glycoprotein containing two major subunits, with Mr values of 75,000 and 37,000.
摘要
树眼镜蛇毒素是一种蛇多肽，它能选择性且强效地阻断神经系统中某些电压敏感的快速激活钾通道，在该系统中它与膜受体具有高亲和力结合。在此，牛突触膜中树眼镜蛇毒素的受体蛋白以活性形式溶解，并通过亲和层析实现了其完全纯化，其中涉及一种新颖的洗脱程序。这种假定的钾通道成分被证明是一种大型寡聚糖蛋白，包含两个主要亚基，其分子量分别为75,000和37,000。
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