Bruns R F, Lawson-Wendling K, Pugsley T A
Anal Biochem. 1983 Jul 1;132(1):74-81. doi: 10.1016/0003-2697(83)90427-x.
Ligand bound to detergent-solubilized or cytosolic receptors can be separated from free ligand by filtration through glass-fiber filters which have been pretreated with polyethylenimine (PEI). Receptors which can be assayed by this technique include detergent-solubilized muscarinic, adenosine A1, alpha 1-adrenergic, alpha 2-adrenergic, beta-adrenergic, dopamine D2, opiate, bradykinin, and benzodiazepine receptors as well as naturally soluble estradiol receptors. For muscarinic, adenosine, alpha 2, dopamine, and estradiol receptors, specific binding measured by the PEI-filter technique was 84-110% of specific binding measured by gel filtration, demonstrating that the technique gave almost quantitative recovery of bound ligand.
与去污剂溶解或胞质受体结合的配体，可通过经聚乙烯亚胺（PEI）预处理的玻璃纤维滤器过滤，与游离配体分离。可通过该技术检测的受体包括去污剂溶解的毒蕈碱、腺苷A1、α1-肾上腺素能、α2-肾上腺素能、β-肾上腺素能、多巴胺D2、阿片、缓激肽和苯二氮䓬受体以及天然可溶的雌二醇受体。对于毒蕈碱、腺苷、α2、多巴胺和雌二醇受体，通过PEI滤器技术测得的特异性结合为通过凝胶过滤测得的特异性结合的84%-110%，表明该技术能几乎定量回收结合的配体。
