The Repetitive Element RLEP Is a Highly Specific Target for Detection of Mycobacterium leprae.
作者信息
Braet S, Vandelannoote K, Meehan C J, Brum Fontes A N, Hasker E, Rosa P S, Lucena-Silva N, Rigouts L, Suffys P N, De Jong B C
机构信息
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.
出版信息
J Clin Microbiol. 2018 Feb 22;56(3). doi: 10.1128/JCM.01924-17. Print 2018 Mar.
Abstract
摘要
相似文献
1
The Repetitive Element RLEP Is a Highly Specific Target for Detection of Mycobacterium leprae.重复元件RLEP是检测麻风分枝杆菌的高度特异性靶点。
J Clin Microbiol. 2018 Feb 22;56(3). doi: 10.1128/JCM.01924-17. Print 2018 Mar.
2
Novel PCR primers for improved detection of Mycobacterium leprae and diagnosis of leprosy.新型 PCR 引物可提高麻风分枝杆菌检测和麻风病诊断的效果。
J Appl Microbiol. 2020 Jun;128(6):1814-1819. doi: 10.1111/jam.14592. Epub 2020 Feb 10.
3
Development of a combined RLEP/16S rRNA (RT) qPCR assay for the detection of viable M. leprae from nasal swab samples.建立一种联合 RLEP/16S rRNA（RT）qPCR 检测方法，用于从鼻拭子样本中检测活的麻风分枝杆菌。
BMC Infect Dis. 2019 Aug 28;19(1):753. doi: 10.1186/s12879-019-4349-9.
4
Comparison of two different PCR amplification products (the 18-kDa protein gene vs. RLEP repetitive sequence) in the diagnosis of Mycobacterium leprae.两种不同PCR扩增产物（18-kDa蛋白基因与RLEP重复序列）在麻风分枝杆菌诊断中的比较。
Clin Exp Dermatol. 2003 Jul;28(4):420-4. doi: 10.1046/j.1365-2230.2003.01300.x.
5
Development of a novel loop-mediated isothermal amplification assay for rapid detection of Mycobacterium leprae in clinical samples.开发一种新型环介导等温扩增检测方法，用于快速检测临床样本中的麻风分枝杆菌。
Indian J Dermatol Venereol Leprol. 2021;87(4):491-497. doi: 10.25259/IJDVL_248_19.
6
Dominant marker (inter-simple sequence repeat-polymerase chain reaction) versus codominant marker (RLEP-polymerase chain reaction) for laboratory diagnosis of leprosy: A comparative evaluation.优势标记（ISSR-PCR）与共显性标记（RLEP-PCR）用于麻风病实验室诊断的比较评估。
Int J Mycobacteriol. 2020 Jan-Mar;9(1):18-23. doi: 10.4103/ijmy.ijmy_190_19.
7
RLEP LAMP for the laboratory confirmation of leprosy: towards a point-of-care test.利福平耐药实时荧光 LAMP 检测试剂盒用于麻风病的实验室确诊：迈向即时检测。
BMC Infect Dis. 2021 Nov 25;21(1):1186. doi: 10.1186/s12879-021-06882-2.
8
Development and validation of a multiplex real-time qPCR assay using GMP-grade reagents for leprosy diagnosis.使用 GMP 级试剂开发和验证用于麻风病诊断的多重实时 qPCR 检测方法。
PLoS Negl Trop Dis. 2022 Feb 18;16(2):e0009850. doi: 10.1371/journal.pntd.0009850. eCollection 2022 Feb.
9
Develop and Field Evolution of Single Tube Nested PCR, SYBRGreen PCR Methods, for the Diagnosis of Leprosy in Paraffin-embedded Formalin Fixed Tissues in Yunnan Province, a Hyper endemic Area of Leprosy in China.建立并现场应用单管巢式 PCR 和 SYBRGreen PCR 方法检测云南省石蜡包埋福尔马林固定组织中麻风分枝杆菌
PLoS Negl Trop Dis. 2019 Oct 2;13(10):e0007731. doi: 10.1371/journal.pntd.0007731. eCollection 2019 Oct.
10
Molecular detection of Mycobacterium leprae in the lachrymal film of leprosy cases, health staff, and healthy individuals.麻风病例、卫生工作人员和健康个体泪膜中麻风分枝杆菌的分子检测。
J Infect Dev Ctries. 2025 Mar 31;19(3):424-430. doi: 10.3855/jidc.20283.
引用本文的文献
1
qPCR detection of DNA in urine samples of leprosy patients using the gene target.使用该基因靶点对麻风病患者尿液样本中的DNA进行qPCR检测。
Front Mol Biosci. 2024 Aug 13;11:1435679. doi: 10.3389/fmolb.2024.1435679. eCollection 2024.
2
Challenges and advances in serological and molecular tests to aid leprosy diagnosis.血清学和分子检测在麻风病诊断中的挑战和进展。
Exp Biol Med (Maywood). 2023 Nov;248(22):2083-2094. doi: 10.1177/15353702231209422. Epub 2023 Dec 7.
3
Highly sensitive and rapid determination of Mycobacterium leprae based on real-time multiple cross displacement amplification.基于实时多重交叉置换扩增的麻风分枝杆菌高灵敏快速检测
BMC Microbiol. 2023 Sep 28;23(1):272. doi: 10.1186/s12866-023-03004-7.
4
Qua vadis leprosy?麻风病将走向何方？
Indian J Med Res. 2023 Jan;157(1):5-10. doi: 10.4103/ijmr.ijmr_2641_22.
5
Mycobacterium leprae Infection in a Wild Nine-Banded Armadillo, Nuevo León, Mexico.墨西哥新莱昂州野生九带犰狳感染麻风分枝杆菌。
Emerg Infect Dis. 2022 Mar;28(3):747-749. doi: 10.3201/eid2803.211295.
6
RLEP LAMP for the laboratory confirmation of leprosy: towards a point-of-care test.利福平耐药实时荧光 LAMP 检测试剂盒用于麻风病的实验室确诊：迈向即时检测。
BMC Infect Dis. 2021 Nov 25;21(1):1186. doi: 10.1186/s12879-021-06882-2.
7
Minimally invasive sampling to identify leprosy patients with a high bacterial burden in the Union of the Comoros.在科摩罗联盟，采用微创取样方法来识别细菌载量高的麻风病患者。
PLoS Negl Trop Dis. 2021 Nov 10;15(11):e0009924. doi: 10.1371/journal.pntd.0009924. eCollection 2021 Nov.
8
Genomic Characterization of to Explore Transmission Patterns Identifies New Subtype in Bangladesh.通过基因组特征分析探索传播模式，在孟加拉国发现新亚型
Front Microbiol. 2020 Jun 16;11:1220. doi: 10.3389/fmicb.2020.01220. eCollection 2020.
9
Isolation of Mycobacterium lepromatosis and Development of Molecular Diagnostic Assays to Distinguish Mycobacterium leprae and M. lepromatosis.麻风分枝杆菌的分离及其分子诊断检测方法的建立，以区分麻风分枝杆菌和麻风分枝杆菌。
Clin Infect Dis. 2020 Nov 5;71(8):e262-e269. doi: 10.1093/cid/ciz1121.
10
Detection of Mycobacterium leprae DNA in soil: multiple needles in the haystack.检测土壤中的麻风分枝杆菌 DNA：大海捞针。
Sci Rep. 2019 Feb 28;9(1):3165. doi: 10.1038/s41598-019-39746-6.
本文引用的文献
1
The new phylogeny of the genus Mycobacterium: The old and the news.分枝杆菌属的新系统发育：旧的和新的。
Infect Genet Evol. 2017 Dec;56:19-25. doi: 10.1016/j.meegid.2017.10.013. Epub 2017 Oct 11.
2
Genomic characterization of Nontuberculous Mycobacteria.非结核分枝杆菌的基因组特征。
Sci Rep. 2017 Mar 27;7:45258. doi: 10.1038/srep45258.
3
Validation of qPCR Methods for the Detection of Mycobacterium in New World Animal Reservoirs.用于检测新大陆动物宿主中分枝杆菌的定量聚合酶链反应方法的验证
PLoS Negl Trop Dis. 2015 Nov 16;9(11):e0004198. doi: 10.1371/journal.pntd.0004198. eCollection 2015 Nov.
4
Evaluation of qPCR-based assays for leprosy diagnosis directly in clinical specimens.基于 qPCR 的检测方法在临床标本中直接用于麻风病诊断的评估。
PLoS Negl Trop Dis. 2011 Oct;5(10):e1354. doi: 10.1371/journal.pntd.0001354. Epub 2011 Oct 11.
5
Enumeration of Mycobacterium leprae using real-time PCR.实时聚合酶链反应检测麻风分枝杆菌。
PLoS Negl Trop Dis. 2008;2(11):e328. doi: 10.1371/journal.pntd.0000328. Epub 2008 Nov 4.
6
Massive gene decay in the leprosy bacillus.麻风杆菌中的大规模基因衰退。
Nature. 2001 Feb 22;409(6823):1007-11. doi: 10.1038/35059006.
7
Basic local alignment search tool.基本局部比对搜索工具
J Mol Biol. 1990 Oct 5;215(3):403-10. doi: 10.1016/S0022-2836(05)80360-2.
Zotero 插件