Brady P S, Brady L J
Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108.
Biochem Pharmacol. 1989 Mar 1;38(5):811-4. doi: 10.1016/0006-2952(89)90235-9.
Clofibrate and acetylsalicylic and have both been reported to increase carnitine palmitoyltransferase (CPT) activity when administered to rats. The purpose of the present study was to determine the mechanism of the increase in CPT activity. Rats (150-200 g) were fed one of the following: chow, chow with 0.5% clofibrate, or chow with 1% acetylsalicylic acid for 2 weeks. At the end of this time, hepatic CPT activity was increased 4-fold over control in the clofibrate group and 3.6-fold over control in the acetylsalicylic acid group. Immunoreactive protein increased 4.0- and 3.6-fold, respectively, over control. Transcription rates of hepatic nuclei were increased 2.8- and 1.9-fold over control in the clofibrate and acetylsalicylic acid groups, and hepatic mRNA levels increased 2.8- and 2.0-fold respectively. These data indicate that increases in CPT activity caused by clofibrate and acetylsalicylic acid administration are due, at least in part, to increased CPT protein, resulting from increased transcription rate and levels of mRNA specific for CPT.
据报道，氯贝丁酯和乙酰水杨酸在给予大鼠时均会增加肉碱棕榈酰转移酶（CPT）的活性。本研究的目的是确定CPT活性增加的机制。给体重150 - 200克的大鼠喂食以下食物之一：普通饲料、含0.5%氯贝丁酯的普通饲料或含1%乙酰水杨酸的普通饲料，持续2周。在此时间段结束时，氯贝丁酯组的肝脏CPT活性比对照组增加了4倍，乙酰水杨酸组比对照组增加了3.6倍。免疫反应性蛋白分别比对照组增加了4.0倍和3.6倍。氯贝丁酯组和乙酰水杨酸组肝细胞核的转录率分别比对照组增加了2.8倍和1.9倍，肝脏mRNA水平分别增加了2.8倍和2.0倍。这些数据表明，给予氯贝丁酯和乙酰水杨酸导致的CPT活性增加至少部分是由于CPT蛋白增加，而这是由CPT特异性mRNA的转录率和水平增加所致。
