Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, College of Life Sciences, Hubei University, Wuhan, Hubei 430062, PR China.
Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, College of Life Sciences, Hubei University, Wuhan, Hubei 430062, PR China; Hubei Tianzhong Stockbreeding Co. Ltd., Wuhan, Hubei 430344, PR China.
Vaccine. 2018 Feb 1;36(6):788-793. doi: 10.1016/j.vaccine.2017.12.072. Epub 2018 Jan 3.
Streptococcus equi ssp. zooepidemicus (SEZ) is an important pathogen of swine streptococcal diseases and can infect a wide range of animals as well as human beings. The absence of effective vaccine confounds the control of SEZ infection. Sec_205, a novel protein identified in the previous study, was inducibly over-expressed in Escherichia coli in the present study. The purified recombinant protein could elicit a significant humoral antibody response and provide efficient protection against lethal challenge of SEZ C55138 in mouse model. The protection against SEZ infection was mediated by specific antibodies to Sec_205 to some extent and was identified by the passive protection assay. The Sec_205 was an in vivo-induced antigen confirmed by the real-time PCR and could adhere to the Hep-2 cells by the inhibition assay. These suggest that Sec_205 may play a vital role in pathogenicity and serve as a new vaccine candidate against SEZ infection.
马链球菌兽疫亚种（SEZ）是一种重要的猪链球菌病病原体，可感染多种动物和人类。由于缺乏有效的疫苗，SEZ 感染的控制变得复杂。本研究中，Sec_205 是在上一个研究中鉴定的一种新型蛋白，在大肠杆菌中可诱导表达。纯化的重组蛋白可诱导产生显著的体液抗体应答，并为 SEZ C55138 在小鼠模型中的致死性攻击提供有效的保护。针对 SEZ 感染的保护作用在一定程度上是通过针对 Sec_205 的特异性抗体介导的，并且通过被动保护试验进行了鉴定。Sec_205 是通过实时 PCR 确认的体内诱导抗原，并且可以通过抑制试验黏附 Hep-2 细胞。这些表明 Sec_205 可能在致病性中发挥重要作用，并可作为针对 SEZ 感染的新型疫苗候选物。
