Tang Bin, Liang Huihuang, Gao Xuejiao, Yan Lili, Deng Mingyong, Zhai Pan, Yang Huahao, Wei Zigong
Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, College of Life Sciences, Hubei University, Wuhan, Hubei 430062, PR China; Environmental Microbial Technology Center of Hubei Province, College of Life Sciences, Hubei University, Wuhan, Hubei 430062, PR China.
Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, College of Life Sciences, Hubei University, Wuhan, Hubei 430062, PR China; Environmental Microbial Technology Center of Hubei Province, College of Life Sciences, Hubei University, Wuhan, Hubei 430062, PR China; Hubei Tianzhong Stockbreeding Co. Ltd., Wuhan, Hubei 430344, PR China.
Res Vet Sci. 2019 Jun;124:387-392. doi: 10.1016/j.rvsc.2019.04.021. Epub 2019 Apr 30.
Streptococcus equi subspecies zooepidemicus (SEZ) is a zoonotic pathogen with adhesive and invasive properties. Due to the shortcomings of antibiotics and traditional inactivated vaccine, identifying protective antigens against SEZ would be helpful to the development of novel vaccines. MAP has been identified as a membrane anchored protein with a typical LPXTG-like cell wall-anchored motif. In present study, the objective was to evaluate the effects of MAP as a subunit vaccine with mouse model. The Western blot analysis shown that the purified recombinant MAP presented good immunoreactive to convalescent porcine sera against SEZ. The protein could elicit a remarkable humoral antibody response and protect 80% of mice against lethal dose challenge of SEZ in mouse model. Moreover, the hyperimmune sera against MAP could efficiently kill the bacteria in whole blood killing assay and conferred significant protection against SEZ in passive immunization experiments. This study suggests with good reasons that MAP could be a novel and effective vaccine candidate for SEZ.
马链球菌兽疫亚种（SEZ）是一种具有黏附性和侵袭性的人畜共患病原体。由于抗生素和传统灭活疫苗存在的缺点，鉴定针对SEZ的保护性抗原将有助于新型疫苗的开发。MAP已被鉴定为一种具有典型LPXTG样细胞壁锚定基序的膜锚定蛋白。在本研究中，目的是用小鼠模型评估MAP作为亚单位疫苗的效果。蛋白质印迹分析表明，纯化的重组MAP对SEZ康复猪血清具有良好的免疫反应性。该蛋白可引发显著的体液抗体反应，并在小鼠模型中保护80%的小鼠免受SEZ致死剂量的攻击。此外，抗MAP的超免疫血清在全血杀菌试验中可有效杀灭细菌，并在被动免疫实验中对SEZ提供显著保护。本研究有充分理由表明，MAP可能是一种新型有效的SEZ疫苗候选物。
