Postgraduate Program, School of Dentistry, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil.
Department of Specific Formation, School of Dentistry, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil.
J Endod. 2018 Mar;44(3):355-362. doi: 10.1016/j.joen.2017.11.001. Epub 2018 Jan 3.
Genetic polymorphisms may result in altered gene expression or functional changes of the encoded molecules and could possibly generate a deficient immunity. Consequently, individuals with specific genotypes could be more susceptible to disease or could present an increase in disease severity. Our study is aimed to verify, through a systematic review and meta-analysis registered in the PROSPERO database (CRD42016043905), whether currently available evidence supports a relationship between interleukin gene polymorphisms and apical periodontitis (AP).
A broad search for studies was conducted. The following databases were used: PubMed, Scopus, Web of Science, and the Virtual Health Library (MEDLINE, SciELO, IBECS, and LILACS). The Medical Subject Headings (MeSH) terms "Periapical Periodontitis," "Periapical Abscess," "Polymorphism, Genetic," and "Polymorphism, Single Nucleotide" were used. MeSH synonyms, related terms, and free terms were included. After application of the eligibility criteria, selected studies were qualified by assessment of their methodologic quality. A fixed effects model was used for the meta-analysis.
The initial search identified 71 references. After excluding duplicate abstracts, 33 were selected. From these, 6 were eligible for quality assessment; 5 were classified as being of moderate quality, and 1 was classified as being of high quality.
From these included studies, polymorphisms in IL1B, IL6, and IL8 were associated with AP. Polymorphisms in IL1A, IL10, or IL12B were not associated with AP regardless of the methodology used. The meta-analysis suggested that the genotype and allele distribution of IL1B (+3954 C/T) gene polymorphism was different in post-treatment AP. More research in this area is warranted to confirm these results.
基因多态性可能导致基因表达改变或编码分子的功能变化，从而导致免疫功能缺陷。因此，具有特定基因型的个体可能更容易患病，或者疾病严重程度可能增加。我们的研究旨在通过在 PROSPERO 数据库（CRD42016043905）中注册的系统评价和荟萃分析来验证，目前的证据是否支持白细胞介素基因多态性与根尖周炎（AP）之间的关系。
广泛搜索研究。使用了以下数据库：PubMed、Scopus、Web of Science 和虚拟健康图书馆（MEDLINE、SciELO、IBECS 和 LILACS）。使用了主题词（MeSH）术语“根尖周炎”、“根尖脓肿”、“遗传多态性”和“单核苷酸多态性”。包括 MeSH 同义词、相关术语和自由术语。应用入选标准后，通过评估研究方法学质量对入选研究进行了合格性评定。荟萃分析采用固定效应模型。
最初的搜索确定了 71 篇参考文献。排除重复摘要后，选择了 33 篇。从这些中，有 6 篇符合质量评估标准；5 篇被归类为中度质量，1 篇被归类为高质量。
从这些纳入的研究中，IL1B、IL6 和 IL8 的多态性与 AP 相关。无论使用何种方法，IL1A、IL10 或 IL12B 的多态性与 AP 无关。荟萃分析表明，IL1B（+3954 C/T）基因多态性的基因型和等位基因分布在治疗后 AP 中不同。需要进一步研究以证实这些结果。
