Department of Stomatology, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China.
National Clinical Research Center for Metabolic Diseases, Hunan Provincial Key Laboratory of Metabolic Bone Diseases, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China.
BMC Oral Health. 2022 Jun 28;22(1):263. doi: 10.1186/s12903-022-02286-z.
The immunological response occurring during periapical inflammation includes expression of nucleotide binding oligomerization domain containing 2 and hepcidin. Nucleotide binding oligomerization domain containing 2 deficiency increases infiltration of inflammatory cells close to alveolar bone. Hepcidin has an important role in iron metabolism affecting bone metabolism.We investigated the role of nucleotide binding oligomerization domain containing 2 and hepcidin in inflammatory periapical periodontitis. Periapical periodontitis was induced in rats and confirmed by micro-computed tomography. Nucleotide binding oligomerization domain 2 and hepcidin were evaluated through immunohistochemistry. Bioinformatics analysis was undertaken usingthe Kyoto Encyclopedia of Genes and Genomes and Gene Ontology databases. Micro-computer tomography revealed alveolar bone resorption in the periapical region and furcation area of mandibular molars in rats of the periapical periodontitis group. Immunohistochemistry showed increased expressionof nucleotide binding oligomerization domain containing 2 and hepcidin around root apices in rats of the periapical periodontitis group. Bioinformatics analysis of differentially expressed genes in inflamed and non-inflamed tissues revealed enrichment in the NOD-like receptor signaling pathway. Our data suggest that nucleotide binding oligomization domain contain2 and hepcidin have important roles in periapical periodontitis severity because they can reduce alveolar bone loss.They could elicit new perspectives for development of novel strategies for periapical periodontitis treatment.
根尖周炎炎症过程中的免疫反应包括核苷酸结合寡聚化结构域 2 和铁调素的表达。核苷酸结合寡聚化结构域 2 缺乏会增加靠近牙槽骨的炎症细胞浸润。铁调素在影响骨代谢的铁代谢中具有重要作用。我们研究了核苷酸结合寡聚化结构域 2 和铁调素在炎症性根尖周炎中的作用。通过 micro-CT 确认在大鼠中诱导了根尖周炎。通过免疫组织化学评估核苷酸结合寡聚化结构域 2 和铁调素。使用京都基因与基因组百科全书和基因本体数据库进行生物信息学分析。Micro-CT 显示根尖周炎组大鼠根尖区和下颌磨牙分叉区牙槽骨吸收。免疫组织化学显示根尖周炎组大鼠根尖周围组织中核苷酸结合寡聚化结构域 2 和铁调素的表达增加。对炎症和非炎症组织中差异表达基因的生物信息学分析显示，NOD 样受体信号通路富集。我们的数据表明，核苷酸结合寡聚化结构域 2 和铁调素在根尖周炎的严重程度中具有重要作用，因为它们可以减少牙槽骨的丢失。它们为开发新的根尖周炎治疗策略提供了新的视角。
