Kussovski Vesselin, Mantareva Vanya, Durmuş Mahmut, Angelov Ivan
The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev, str., Bl. 26, 1113 Sofia, Bulgaria.
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev, str., Bl. 9, 1113 Sofia, Bulgaria, Tel.: +35-92-9606-181.
Z Naturforsch C J Biosci. 2018 Apr 25;73(5-6):221-228. doi: 10.1515/znc-2017-0167.
Photodynamic inactivation (PDI) has been featured as an effective strategy in the treatment of acute drug-resistant infections. The efficiency of PDI was evaluated against three periodontal pathogenic bacteria that were tested as drug-resistant strains. In vitro studies were performed with four water-soluble cationic Zn(II) phthalocyanines (ZnPc1-4) and irradiation of a specific light source (light-emitting diode, 665 nm) with three doses (15, 36 and 60 J/cm2). The well detectable fluorescence of ZnPcs allowed the cellular imaging, which suggested relatively high uptakes of ZnPcs into bacterial species. Complete photoinactivation was achieved with all studied ZnPc1-4 for Enterococcus faecalis (E. faecalis) at a light dose of 15 J/cm2. The photodynamic response was high for Prevotella intermedia (P. intermedia) after the application of 6 μM of ZnPc1 and a light dose of 36 J/cm2 and for 6 μM of ZnPc2 at 60 J/cm2. P. intermedia was inactivated with ZnPc3 (4 log) and ZnPc4 (2 log) with irradiation at an optimal dose of 60 J/cm2. Similar photoinactivation results (2 log) were achieved for Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) treated with 6 μM ZnPc1 and ZnPc2 at a light dose of 60 J/cm2. The study suggested that PDI with quaternized Zn(II) phthalocyanines and specific light irradiation appears to be a very useful antimicrobial strategy for effective inactivation of drug-resistant periodontal pathogens.
光动力灭活（PDI）已成为治疗急性耐药感染的一种有效策略。评估了PDI对三种作为耐药菌株进行测试的牙周病原菌的效果。使用四种水溶性阳离子锌（II）酞菁（ZnPc1 - 4）进行体外研究，并使用特定光源（发光二极管，665 nm）以三种剂量（15、36和60 J/cm²）进行照射。ZnPcs良好的可检测荧光使得细胞成像成为可能，这表明ZnPcs在细菌种类中的摄取相对较高。在15 J/cm²的光剂量下，所有研究的ZnPc1 - 4对粪肠球菌（E. faecalis）均实现了完全光灭活。在应用6 μM的ZnPc1和36 J/cm²的光剂量后，以及在60 J/cm²下应用6 μM的ZnPc2后，中间普氏菌（P. intermedia）的光动力反应较高。在60 J/cm²的最佳照射剂量下，ZnPc3（4个对数）和ZnPc4（2个对数）使中间普氏菌失活。在用6 μM ZnPc1和ZnPc2在60 J/cm²的光剂量下处理后，伴放线聚集杆菌（A. actinomycetemcomitans）也获得了类似的光灭活结果（2个对数）。该研究表明，用季铵化锌（II）酞菁进行PDI和特定光照射似乎是有效灭活耐药牙周病原体的一种非常有用的抗菌策略。
