State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Sci China Life Sci. 2018 Jun;61(6):718-726. doi: 10.1007/s11427-017-9200-1. Epub 2018 Jan 3.
Fungi play important roles in ecosystem processes, and the elevational pattern of fungal diversity is still unclear. Here, we examined the diversity of fungi along a 1,000 m elevation gradient on Mount Nadu, Southwestern China. We used MiSeq sequencing to obtain fungal sequences that were clustered into operational taxonomic units (OTUs) and to measure the fungal composition and diversity. Though the species richness and phylogenetic diversity of the fungal community did not exhibit significant trends with increasing altitude, they were significantly lower at mid-altitudinal sites than at the base. The Bray-Curtis distance clustering also showed that the fungal communities varied significantly with altitude. A distance-based linear model multivariate analysis (DistLM) identified that soil pH dominated the explanatory power of the species richness (23.72%), phylogenetic diversity (24.25%) and beta diversity (28.10%) of the fungal community. Moreover, the species richness and phylogenetic diversity of the fungal community increased linearly with increasing soil pH (P<0.05). Our study provides evidence that pH is an important predictor of soil fungal diversity along elevation gradients in Southwestern China.
真菌在生态系统过程中发挥着重要作用，但其海拔多样性模式仍不清楚。本研究沿中国西南那都山的 1000 米海拔梯度，调查了真菌多样性。我们使用 MiSeq 测序获得真菌序列，并将其聚类为操作分类单元 (OTUs)，以测量真菌组成和多样性。尽管真菌群落的物种丰富度和系统发育多样性随海拔升高没有显著趋势，但在中海拔地区显著低于海拔较低地区。Bray-Curtis 距离聚类也表明，真菌群落随海拔显著变化。基于距离的线性模型多元分析 (DistLM) 确定，土壤 pH 主导了真菌群落物种丰富度 (23.72%)、系统发育多样性 (24.25%) 和β多样性 (28.10%) 的解释能力。此外，真菌群落的物种丰富度和系统发育多样性随土壤 pH 的增加呈线性增加 (P<0.05)。本研究提供了证据，表明 pH 是中国西南沿海拔梯度土壤真菌多样性的重要预测因子。
