Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
Can J Microbiol. 2020 May;66(5):359-367. doi: 10.1139/cjm-2019-0589. Epub 2020 Feb 13.
The magnitude of the impact of altitude gradient on microbial community and diversity has been studied in recent decades. Whereas bacteria have been the focus of most studies, fungi have been given relatively less attention. As a vital part of the macro- and microscopic ecosystem, rhizosphere fungi play a key role in organic matter decomposition and relative abundance of plant species and have an impact on plant growth and development. Using as the host plant, we examined the rhizosphere soil fungal community patterns across the altitude gradient in 15 sites of Yunnan province by sequencing the fungal ITS2 region with the Illumina MiSeq platform. We determined the fungal community composition and structure. We found that, surprisingly, rhizosphere soil fungal diversity of increased with altitudinal gradient. There was a slight difference in diversity between samples from high- and medium-altitude sites, with medium-altitude sites having the greater diversity. Furthermore, the rhizosphere soil fungal community composition and structure kept changing along the altitudinal gradient. Taxonomic results showed that the extent of phylum diversity was greatest at high-altitude sites, with , , , and as the most dominant fungal phyla.
近几十年来,人们研究了海拔梯度对微生物群落和多样性的影响程度。尽管细菌一直是大多数研究的焦点,但真菌相对较少受到关注。作为宏观和微观生态系统的重要组成部分,根际真菌在有机质分解和植物物种相对丰度中起着关键作用,并影响植物的生长和发育。本研究以 为宿主植物,通过 Illumina MiSeq 平台对云南 15 个地点的海拔梯度的根际土壤真菌 ITS2 区进行测序,研究了根际土壤真菌群落模式。我们确定了真菌群落组成和结构。令人惊讶的是, 的根际土壤真菌多样性随海拔梯度增加。高海拔和中海拔地点的多样性略有差异,中海拔地点的多样性更高。此外,根际土壤真菌群落组成和结构沿着海拔梯度不断变化。分类学结果表明,高海拔地点的门多样性最大,最主要的真菌门是 、 、 和 。