Yousefi Hila, Najafi Shamsolmoulouk, Mohammadzadeh Mahsa, Zare Bidoki Alireza, Farhadi Elham, Rezaei Nima
Toxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Dental Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Oral Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Acta Med Iran. 2017 Nov;55(11):672-675.
This study has been conducted to evaluate the allele, genotype and haplotype frequencies of the polymorphic gene coding TGF-β in recurrent aphthous stomatitis (RAS). TGF-β gene typing was done by polymerase chain reaction with sequence-specific primers (PCR-SSP) assay. Allele frequencies were estimated by direct gene counting. C allele at codon 25 was significantly increased, while G allele at this position was significantly decreased in patients compared to the controls. A significantly higher frequency of CG genotype at codon 25 was found in control group. CC genotype and TT genotype at codon 10 of the gene was significantly decreased, while CT genotype at the same position was significantly increased in patients, indicating that CT heterozygosity at codon 10 TGF-β is associated with greater risk of RAS. CG and TG haplotypes were significantly decreased while CC and TC haplotypes were significantly increased in patients compared with controls. This study indicates the TGF-β single nucleotide polymorphisms could play a role in RAS pathogenesis. Thereby certain SNPs of TGF-β gene have an association with RAS pathogenesis.
本研究旨在评估复发性阿弗他口炎（RAS）中编码转化生长因子-β（TGF-β）的多态性基因的等位基因、基因型和单倍型频率。采用序列特异性引物聚合酶链反应（PCR-SSP）法进行TGF-β基因分型。通过直接基因计数估计等位基因频率。与对照组相比，患者第25密码子处的C等位基因显著增加，而该位置的G等位基因显著减少。在对照组中发现第25密码子处CG基因型的频率显著更高。该基因第10密码子处的CC基因型和TT基因型在患者中显著减少，而同一位置的CT基因型在患者中显著增加，表明第10密码子处TGF-β的CT杂合性与RAS的更高风险相关。与对照组相比，患者的CG和TG单倍型显著减少，而CC和TC单倍型显著增加。本研究表明，TGF-β单核苷酸多态性可能在RAS发病机制中起作用。因此，TGF-β基因的某些单核苷酸多态性与RAS发病机制相关。
