Bidoki Alireza Zare, Harsini Sara, Sadr Maryam, Soltani Samaneh, Mohammadzadeh Mahsa, Najafi Shamsolmoulouk, Rezaei Nima
Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
J Oral Pathol Med. 2016 Feb;45(2):136-40. doi: 10.1111/jop.12332. Epub 2015 May 28.
Recurrent aphthous stomatitis (RAS) is a common disorder with an unclear etiopathogenesis. Involvement of the immune system in the development of this condition is strongly suggested. As the variations in the inflammasome-related NLRP3 gene have been suggested to affect immune system activity, this case-control study was performed to determine whether these genetic variants are associated with RAS.
We studied a group of 69 Iranian patients with RAS in comparison with 56 healthy controls. We determined four single nucleotide polymorphisms (SNPs) of NLRP3 and performed association analyses of NLRP3. Genotyping was conducted using the TaqMan method.
The NLRP3 rs3806265 T allele was significantly more frequent in the patients with RAS than in the healthy controls (P = 0.003). While a significant negative association was found between the C allele at the same position with RAS (P = 0.003), the TT genotype was significantly more frequent at position rs3806265 in NLRP3 in patient group than in the controls (P = 0.002). However, the frequency of CT genotype at the same position was significantly higher in healthy controls than in the case category (P = 0.002).
Considering the high frequency of the presence of NLRP3 rs3806265 TT genotype in patients with RAS, it seems that this gene polymorphism could affect individual susceptibility to RAS.
复发性阿弗他口炎（RAS）是一种常见疾病，其病因发病机制尚不清楚。强烈提示免疫系统参与了该疾病的发生发展。由于炎性小体相关的NLRP3基因变异被认为会影响免疫系统活性，因此进行了这项病例对照研究，以确定这些基因变异是否与RAS相关。
我们研究了一组69名伊朗RAS患者，并与56名健康对照进行比较。我们确定了NLRP3的四个单核苷酸多态性（SNP），并对NLRP3进行了关联分析。使用TaqMan方法进行基因分型。
RAS患者中NLRP3 rs3806265 T等位基因的频率显著高于健康对照（P = 0.003）。虽然在同一位置的C等位基因与RAS之间发现了显著的负相关（P = 0.003），但患者组中NLRP3 rs3806265位置的TT基因型频率显著高于对照组（P = 0.002）。然而，同一位置的CT基因型频率在健康对照中显著高于病例组（P = 0.002）。
考虑到RAS患者中NLRP3 rs3806265 TT基因型的高频率存在，似乎这种基因多态性可能会影响个体对RAS的易感性。
