Akcora Yildiz Dilara, Irtegun Kandemir Sevgi, Agacayak Elif, Deveci Engin
Department of Biology, Faculty of Science and Arts, Mehmet Akif Ersoy University, Burdur, Turkey.
Department of Medical Biology, Faculty of Medicine, Dicle University, Diyarbakir, Turkey.
Cell Mol Biol (Noisy-le-grand). 2017 Dec 30;63(12):51-55. doi: 10.14715/cmb/2017.63.12.12.
Preeclampsia is a severe multisystem disorder, and its pathophysiology is still not completely understood. Autophagy, a recycling process that maintains cellular homoeostasis during differentiation and development, is controversial regarding increased or decreased autophagic activity in preeclampsia. The aim of this study was to determine whether autophagy is increased in the placentas of women with preeclampsia by examining the protein levels of autophagy markers (Beclin 1 and SQSTM1/p62) and phosphorylation of cyclin E. For this purpose, placentas from preeclampsia (n=10) and control (n=10) pregnancies were included in this study. The protein expression of autophagy-related markers Beclin1, SQSTM1/p62 and phosphorylation status of cyclin E were detected by Western blot. Our data showed that the protein levels of both Beclin 1 and SQSTM1/p62 were significantly increased, while the phosphorylation level of cyclin E was significantly decreased in placentas with preeclampsia compared to those derived from controls. The results of this study suggest that the autophagic activity is perpetually increased in preeclampsia and cyclin E protein stabilisation might be involved in the induction of autophagy.
子痫前期是一种严重的多系统疾病，其病理生理学仍未完全明确。自噬是一种在分化和发育过程中维持细胞稳态的循环过程，子痫前期中自噬活性是增加还是降低存在争议。本研究的目的是通过检测自噬标志物（Beclin 1和SQSTM1/p62）的蛋白水平以及细胞周期蛋白E的磷酸化情况，来确定子痫前期患者胎盘组织中的自噬是否增强。为此，本研究纳入了子痫前期孕妇（n = 10）和对照孕妇（n = 10）的胎盘组织。通过蛋白质免疫印迹法检测自噬相关标志物Beclin1、SQSTM1/p62的蛋白表达以及细胞周期蛋白E的磷酸化状态。我们的数据显示，与对照组胎盘组织相比，子痫前期胎盘组织中Beclin 1和SQSTM1/p62的蛋白水平均显著升高，而细胞周期蛋白E的磷酸化水平则显著降低。本研究结果提示，子痫前期患者的自噬活性持续增强，细胞周期蛋白E的蛋白稳定可能参与了自噬的诱导过程。
