Siddiqui Samra, Saeed Mohd, Faisal Syed Mohd, Akhtar Asma, Sarim Khan Mohd, Khan Mahvish, Ahmad Israr, Ahmad Irfan
Department of Biosciences (Microbiology), Integral University, Lucknow, UP, India.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Hail, Hail, KSA.
Cell Mol Biol (Noisy-le-grand). 2017 Dec 30;63(12):63-67. doi: 10.14715/cmb/2017.63.12.14.
Gram-positive bacteria, particularly Staphylococcus aureus is a significant pathogen, not only in the hospital setting but the community also. S. aureus is a major cause of serious hospital and community-acquired infections, particularly in the colonized individuals. The emergence of vancomycin-resistant S. aureus (VRSA) strains has led to global concerns about treatments for staphylococcal infections. Until now, few strains of VRSA have been reported worldwide. The conventional disk diffusion method for determination of vancomycin sensitivity often misclassifies intermediately susceptible isolates to fully sensitive. However, non-automated minimum inhibitory concentration (MIC) detection methods are the gold standards. Hence there is a dire need of some advanced methods for rapid detection of VRSA strains. In the present study, Gram-positive clinical isolates were collected from different wards of K.G.M.U. Hospital, among them, 12 bacterial isolates were identified as Staphylococcus aureus and 18 isolates as Klebsiella spp. Genomic DNA of S. aureus was isolated and used as template in PCR for detection of the presence of van A and van X gene based on a given protocol. Nosocomial infections have an impact on morbidity and probably on mortality as well, and pose a significant economic burden. Rapid molecular identification of antibiotic-resistant strains undoubtedly helps to prevent the hospital-induced infections.
革兰氏阳性菌，尤其是金黄色葡萄球菌，不仅在医院环境中，在社区环境中也是一种重要的病原体。金黄色葡萄球菌是严重医院感染和社区获得性感染的主要原因，尤其是在定植个体中。耐万古霉素金黄色葡萄球菌（VRSA）菌株的出现引发了全球对葡萄球菌感染治疗的关注。到目前为止，全球报道的VRSA菌株很少。用于测定万古霉素敏感性的传统纸片扩散法常常将中介敏感菌株误分类为完全敏感。然而，非自动化的最低抑菌浓度（MIC）检测方法是金标准。因此，迫切需要一些先进的方法来快速检测VRSA菌株。在本研究中，从K.G.M.U.医院的不同病房收集革兰氏阳性临床分离株，其中，12株细菌分离株被鉴定为金黄色葡萄球菌，18株分离株为克雷伯菌属。分离出金黄色葡萄球菌的基因组DNA，并将其用作PCR模板，根据给定方案检测van A和van X基因的存在。医院感染对发病率有影响，可能对死亡率也有影响，并带来重大的经济负担。对抗生素耐药菌株进行快速分子鉴定无疑有助于预防医院感染。
