Climate Change Cluster, University of Technology Sydney, NSW, Australia; Centre for Tropical Water and Aquatic Ecosystem Research (TropWater), James Cook University, Cairns, QLD, Australia.
Climate Change Cluster, University of Technology Sydney, NSW, Australia.
Mar Environ Res. 2018 Mar;134:55-67. doi: 10.1016/j.marenvres.2017.12.012. Epub 2017 Dec 24.
In this study we investigated the effect of light-limitation (∼20 μmol photons m s) on the southern hemisphere seagrass, Zostera muelleri. RNA sequencing, chlorophyll fluorometry and HPLC techniques were used to investigate how the leaf-specific transcriptome drives changes in photosynthesis and photo-pigments in Z. muelleri over 6 days. 1593 (7.51%) genes were differentially expressed on day 2 and 1481 (6.98%) genes were differentially expressed on day 6 of the experiment. Differential gene expression correlated with significant decreases in rETR, I, an increase in Yi (initial photosynthetic quantum yield of photosystem II), and significant changes in pigment composition. Regulation of carbohydrate metabolism was observed along with evidence that abscisic acid may serve a role in the low-light response of this seagrass. This study provides a novel understanding of how Z. muelleri responds to light-limitation in the marine water column and provides potential molecular markers for future conservation monitoring efforts.
在这项研究中，我们研究了光限制（约 20µmol 光子 m-2 s-1）对南半球海草 Zostera muelleri 的影响。使用 RNA 测序、叶绿素荧光计和 HPLC 技术，研究了叶片特异性转录组如何在 6 天内驱动 Z. muelleri 光合作用和光色素的变化。在实验的第 2 天和第 6 天，有 1593 个（7.51%）基因差异表达，有 1481 个（6.98%）基因差异表达。差异基因表达与 rETR、I 的显著下降以及 Yi（光系统 II 初始光合量子产量）的增加相关，并且色素组成也发生了显著变化。观察到碳水化合物代谢的调节，并有证据表明脱落酸可能在这种海草对低光的反应中起作用。本研究提供了对 Z. muelleri 如何响应海洋水柱中光限制的新认识，并为未来的保护监测工作提供了潜在的分子标记。
