Laboratorio de Investigación de Productos Naturales (LIPRON), Instituto de Bioprospección y Fisiología Vegetal (INBIOFIV-CONICET), Facultad de Ciencias Naturales e IML, Universidad Nacional de Tucumán, San Lorenzo 1469 San Miguel de Tucumán, Tucumán, Argentina.
INEI-ANLIS 'Dr. Carlos G. Malbrán', Departamento de Micología, Av. Vélez Sarsfield 563, Ciudad Autónoma de Buenos Aires, Argentina.
J Ethnopharmacol. 2018 Apr 24;216:283-294. doi: 10.1016/j.jep.2018.01.001. Epub 2018 Jan 4.
The Argentinean medicinal plant Tetraglochin andina Ciald, formerly classified as T. cristatum (Britton) Rothm is used in traditional medicine by inhabitants from Argentinean northwestern highlands (Puna) to treat candidiasis and as anti-inflammatory.
To assess the potential of the crude drug as an anti-Candida agent with anti-inflammatory properties. The bioactivity and phytochemical composition of a dry extract of the plant was investigated.
The pharmacognostic description of the crude drug is carried out for the first time, including macroscopic and microscopic examinations of the different organs, physicochemical and extractive values (petroleum ether-, ethanol- and water-soluble). The dry extract from T. andina was evaluated as antifungal against pathogenic Candida sp. and Saccharomyces cerevisiae isolated from vaginal infections and reference strains, by the macrodilution and microdilution assays. The normal vaginal microbiome in women is characterized by the dominance of lactic acid-producing bacteria, mainly Lactobacillus spp. The effect of T. andina extract on Lactobacillus strains was also assayed. The inhibitory effect on proinflammatory enzymes (cyclooxygenase, lipoxygenase and phospholipase A) and antioxidant capacity was studied. The chemical profile was analyzed by HPLC-ESI-MS.
The hydroalcoholic extract inhibited the growth of all yeasts with Minimal Inhibitory Concentration (MIC) values between 12.5 and 400 µg GAE/mL and the MIC values on Lactobacillus were higher than the MIC values against Candida isolates ( > 400 µg GAE/mL). These results indicate that the hydroalcoholic extract could be used without affecting the normal microbiota of vaginal fluid. The extract showed antioxidant activity and could modulate the inflammatory process by three pathways (sPLA, COX-2, LOX). The plant extract contained high total phenolic levels (386.9±1.7 mg GAE/g dry extract) and flavonoid levels (260.4±2.7 mg GAE/g dry extract). Fifty phenolic compounds were identified by HPLC-ESI-MS. They were mainly hydrolysable and condensed tannins. The dry extract was chemically and biologically stable during one year at room temperature or 4 °C.
The presence of anti-Candida and anti-inflammatory activities in Tetraglochin andina extracts give support to their traditional use for treating conditions associated with microorganism infections and inflammatory process in humans. This plant preparation could be used to design phytopharmaceutical preparations to inhibit yeast growth and moderate the inflammatory and oxidative process.
阿根廷药用植物 Tetraglochin andina Ciald，以前被归类为 T. cristatum (Britton) Rothm，被阿根廷西北部高原（普纳）的居民用于传统医学，用于治疗念珠菌病和抗炎。
评估该生药作为具有抗炎特性的抗念珠菌剂的潜力。研究了该植物的干提取物的生物活性和植物化学成分。
首次对生药进行了生药学描述，包括不同器官的宏观和微观检查、理化和浸出值（石油醚、乙醇和水可溶）。用微量稀释法和微量稀释法评估来自阴道感染和参考菌株的致病性念珠菌和酿酒酵母的抗真菌活性。阴道正常微生物群由产乳酸细菌（主要是 Lactobacillus spp.）主导。还测定了 Tetraglochin andina 提取物对乳酸杆菌菌株的作用。研究了对促炎酶（环加氧酶、脂加氧酶和磷脂酶 A）和抗氧化能力的抑制作用。通过 HPLC-ESI-MS 分析了化学特征。
水醇提取物抑制所有酵母的生长，最小抑菌浓度（MIC）值在 12.5 至 400 μg GAE/mL 之间，对 Lactobacillus 的 MIC 值高于对念珠菌分离株的 MIC 值（>400 μg GAE/mL）。这些结果表明，该水醇提取物可在不影响阴道液正常微生物群的情况下使用。提取物具有抗氧化活性，并可通过三种途径（sPLA、COX-2、LOX）调节炎症过程。植物提取物含有高总酚水平（386.9±1.7 μg GAE/g 干提取物）和类黄酮水平（260.4±2.7 μg GAE/g 干提取物）。通过 HPLC-ESI-MS 鉴定了 50 种酚类化合物。它们主要是可水解和缩合单宁。在室温或 4°C 下，干提取物在一年的时间内保持化学和生物稳定性。
Tetraglochin andina 提取物具有抗念珠菌和抗炎活性，为其传统用于治疗与微生物感染和人类炎症过程相关的疾病提供了支持。这种植物制剂可用于设计植物药制剂，以抑制酵母生长并调节炎症和氧化过程。
