Laboratorio de Investigación de Productos Naturales (LIPRON), Instituto de Bioprospección y Fisiología Vegetal (INBIOFIV-CONICET-UNT), Argentina.
Laboratorio de Investigación de Productos Naturales (LIPRON), Instituto de Bioprospección y Fisiología Vegetal (INBIOFIV-CONICET-UNT), Argentina; Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucumán, San Lorenzo 1469, San Miguel de Tucumán, Tucumán, Argentina.
J Ethnopharmacol. 2020 May 10;253:112642. doi: 10.1016/j.jep.2020.112642. Epub 2020 Feb 5.
Argentinean medicinal plants such as Larrea divaricata Cav., Larrea cuneifolia Cav., Larrea nitida Cav., Zuccagnia punctata Cav. and Tetraglochin andina Ciald. are used alone and in combination in traditional medicine by inhabitants from northwestern Argentina to solve mycosis, vaginal infections, gastrointestinal, respiratory, and inflammatory processes.
To assess the effect of interactions between hydroalcoholic extracts of these five species of medicinal plants against yeast strains isolated from vaginal infections, select the most active mixtures and evaluate anti-inflammatory and antioxidant activities.
Synergy between the plant extracts was studied by using a broth microdilution assay by means of the checkerboard method against Saccharomyces cerevisiae, Candida albicans, and non albicans strains. The inhibitory effect on lipoxygenase and the antioxidant capacity in cell-free and cell systems were studied. The chemical profile was evaluated by qualitative and quantitative screening, whereas chemical markers were quantified by HPLC-DAD.
A synergistic antifungal effect was observed in some binary combinations. Z. punctata/L. divaricata, Z. punctata/L. cuneifolia, and Z. punctata/L. nitida were the most active mixtures. Nordihydroguaiaretic acid and 2',4'-dihydroxychalcone, two antifungal compounds, present in these extracts, were identified and quantified by HPLC-DAD. Both single extracts and bi-herbal mixtures showed antioxidant activity (in cellular and in cell-free systems) and were active on pro-inflammatory enzymes (LOX).
Our results indicated that the most active combinations of these species extracts could be useful in the treatment of vaginal infectious diseases caused by Saccharomyces cerevisiae and Candida spp. strains and in associated oxidative and inflammatory processes, supporting its traditional use. In addition, the results highlighted the phyto-therapeutic potential of total phytochemical compounds present in these medicinal plants.
阿根廷药用植物，如 Larrea divaricata Cav.、Larrea cuneifolia Cav.、Larrea nitida Cav.、Zuccagnia punctata Cav. 和 Tetraglochin andina Ciald.，被来自阿根廷西北部的居民单独或组合使用于传统医学中，以解决真菌感染、阴道感染、胃肠道、呼吸和炎症过程。
评估这五种药用植物的水醇提取物之间相互作用对阴道感染分离酵母株的影响，选择最有效的混合物，并评估抗炎和抗氧化活性。
通过棋盘微量稀释法，使用比浊法研究植物提取物之间的协同作用，对抗酿酒酵母、白色念珠菌和非白色念珠菌菌株。研究了对脂氧合酶的抑制作用和细胞内外的抗氧化能力。通过定性和定量筛选评估化学特征，通过 HPLC-DAD 定量化学标志物。
观察到一些二元组合具有协同抗真菌作用。Z. punctata/L. divaricata、Z. punctata/L. cuneifolia 和 Z. punctata/L. nitida 是最有效的混合物。这些提取物中存在的两种具有抗真菌作用的化合物，即 Nordihydroguaiaretic acid 和 2',4'-dihydroxychalcone，通过 HPLC-DAD 进行了鉴定和定量。单一提取物和双草药混合物均表现出抗氧化活性（在细胞内和细胞外系统），并对促炎酶（LOX）有活性。
我们的结果表明，这些物种提取物的最有效组合可能对治疗由酿酒酵母和念珠菌属菌株引起的阴道感染性疾病以及相关的氧化和炎症过程有用，支持其传统用途。此外，这些结果突出了这些药用植物中存在的总植物化学化合物的植物治疗潜力。
