Post-Graduate Department of Biosciences, UGC-Centre of Advanced Study, Satellite Campus, Vadtal Road, Sardar Patel University, Bakrol, Anand 388315, Gujarat, India.
Post-Graduate Department of Biosciences, UGC-Centre of Advanced Study, Satellite Campus, Vadtal Road, Sardar Patel University, Bakrol, Anand 388315, Gujarat, India; School of Sciences, P. P. Savani University, Dhamdod, Kosamba 394125, Gujarat, India.
Int J Biol Macromol. 2018 May;111:359-369. doi: 10.1016/j.ijbiomac.2017.12.170. Epub 2018 Jan 5.
In the present study, blue light absorbing pigment protein phycoerythrin (PE) is purified up to molecular grade purity from marine Halomicronema sp. R31DM. The purification method is based on the use of non-ionic detergent Triton-X 100 in ammonium sulphate precipitation. The purified PE is characterized for its antioxidant activity in vitro and in vivo. PE is noted to show substantial in vitro antioxidant activity probed by various biochemical assays. The PE moderated rise in the intracellular-ROS (reactive oxygen species) in wild type Caenorhabditis elegans upon heat and oxidative stress. Further, the antioxidant asset of PE is noted an expedient in averting the ROS associated abnormalities, i.e. impaired physiological behaviour (health span) and aging in C. elegans. The structural attributes of PE contributing to its antioxidant virtue are analysed; the presence of ample residues having antioxidant activity and chromophore-PEB in PE are identified as a source of its antioxidant activity. Furthermore, the stability of PE is assessed under three physico-chemical stresses, temperature, pH and oxidative stress.
在本研究中，从海洋 Halomicronema sp. R31DM 中纯化出达到分子级纯度的蓝光吸收色素蛋白藻红蛋白 (PE)。该纯化方法基于使用非离子型去污剂 Triton-X 100 在硫酸铵沉淀中的应用。纯化的 PE 具有体外和体内抗氧化活性的特征。PE 通过各种生化测定显示出显著的体外抗氧化活性。PE 可调节野生型秀丽隐杆线虫在热和氧化应激下细胞内 ROS（活性氧）的升高。此外，PE 的抗氧化资产可避免与 ROS 相关的异常，即秀丽隐杆线虫的生理行为（健康跨度）和衰老受损。分析了 PE 的结构属性与其抗氧化性能之间的关系；鉴定出 PE 中具有抗氧化活性的大量残基和发色团-PEB 是其抗氧化活性的来源。此外，还评估了 PE 在三种物理化学应激（温度、pH 和氧化应激）下的稳定性。
