单壁碳纳米管探测溶菌酶的变性
Single-Walled Carbon Nanotubes Probing the Denaturation of Lysozyme.
作者信息
Xie Liming, Chou Shin G, Pande Ajay, Pande Jayanti, Zhang Jin, Dresselhaus Mildred S, Kong Jing, Liu Zhongfan
机构信息
Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China, Pfizer Global Research and Development, Pfizer Inc., Groton, Connecticut 06340, Optical Technology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, Chemistry Department, University at Albany, State University of New York, Albany, New York 12222, and Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
出版信息
J Phys Chem C Nanomater Interfaces. 2010 May 6;114(17):7717-7720. doi: 10.1021/jp9121497. Epub 2010 Apr 2.
Resonance Raman spectroscopy measurements of lysozyme-bound single-walled carbon nanotubes have been made during different stages of the chemically and thermally induced misfolding and of the denaturation process of nanotube-bound lysozymes. Changes to the Raman intensity of single-walled carbon nanotubes (SWNTs) have been observed during the denaturation of lysozyme. The Raman intensity changes are attributed to excitonic transition energy ( ) shifts of the SWNTs during the denaturation of lysozyme. The shift of SWNTs was confirmed by photoluminescence measurements.
在化学和热诱导的溶菌酶错误折叠的不同阶段以及与纳米管结合的溶菌酶的变性过程中，对与溶菌酶结合的单壁碳纳米管进行了共振拉曼光谱测量。在溶菌酶变性过程中观察到了单壁碳纳米管（SWNTs）拉曼强度的变化。拉曼强度的变化归因于溶菌酶变性过程中SWNTs的激子跃迁能量（）的变化。SWNTs的变化通过光致发光测量得到了证实。
Zotero 插件