松萝酸对映体的体外抗/抗原毒性活性

Pro/Antigenotoxic Activity of Usnic Acid Enantiomers In Vitro.

作者信息

Prokop'ev I A, Filippov E V, Filippova G V, Zhanataev A K

机构信息

Institute of Biological Problems of Cryolithozone, Siberian Division of the Russian Academy of Sciences, Yakutsk, Sakha Republic, Russia.

V. V. Zakusov Research Institute of Pharmacology, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2018 Jan;164(3):312-315. doi: 10.1007/s10517-018-3979-z. Epub 2018 Jan 8.

DOI:10.1007/s10517-018-3979-z

PMID:29308568

Abstract

The effect of usnic acid enantiomers on the genotoxic effects of dioxidine and methyl methanesulfonate was studied in vitro in human peripheral blood lymphocytes by the DNA comet method. We found that usnic acid enantiomers in a concentration range of 0.01-1.00 μM demonstrated pronounced antigenotoxic activity and reduced DNA damage induced by genotoxicants by 37-70%. In the same concentration range, the test enantiomers reduced the level of atypical DNA comets (hedgehogs) induced by genotoxicants by 23-61%. The test compounds did not modulate the effects of genotoxicants in a concentration of 10 μM and potentiated them in a concentration of 100 μM. The modifying activity of usnic acid did not depend on spatial configuration and on the used model genotoxicant.

摘要

采用DNA彗星试验法，在体外人外周血淋巴细胞中研究了松萝酸对二氧嗪和甲磺酸甲酯遗传毒性的影响。我们发现，浓度范围为0.01 - 1.00 μM的松萝酸对映体表现出显著的抗遗传毒性活性，可使遗传毒性剂诱导的DNA损伤减少37 - 70%。在相同浓度范围内，测试对映体可使遗传毒性剂诱导的非典型DNA彗星（刺猬状）水平降低23 - 61%。测试化合物在10 μM浓度下未调节遗传毒性剂的作用，而在100 μM浓度下增强了其作用。松萝酸的修饰活性不依赖于空间构型和所用的模型遗传毒性剂。