(+)-和(-)-松萝酸对小鼠的遗传毒性

Genotoxicity of (+)- and (-)-usnic acid in mice.

作者信息

Prokopiev Ilya, Filippova Galina, Filippov Eduard, Voronov Ivan, Sleptsov Igor, Zhanataev Aliy

机构信息

Institute for Biological Problems of Cryolithozone, Siberian Brunch of the Russian Academy of Sciences, Lenina 41, Yakutsk 677980, Sakha Republic, Russia.

出版信息

Mutat Res Genet Toxicol Environ Mutagen. 2019 Mar;839:36-39. doi: 10.1016/j.mrgentox.2019.01.010. Epub 2019 Jan 21.

DOI:10.1016/j.mrgentox.2019.01.010

PMID:30744810

Abstract

Usnic acid, which is the most widespread and well-studied secondary lichen compound, has antibacterial and cytotoxic effects. Usnic acid is present in lichens as the (+)- and (-)-enantiomers, which have different biological activities. We used a DNA-comet assay to determine the genotoxic effect of (+)- and (-)-usnic acid in the liver and kidney cells of mice. The genotoxic effect of usnic acid was only observed 1 h after oral administration. Usnic acid doses of 100 and 50 mg/kg resulted in DNA damage in the liver and kidney cells. The genotoxic effect of usnic acid is associated with oxidative stress in cells. There were no significant differences in the effects of (+)- and (-)-enantiomers.

摘要

松萝酸是分布最广且研究最多的地衣次生化合物，具有抗菌和细胞毒性作用。松萝酸在地衣中以（+）-和（-）-对映体形式存在，它们具有不同的生物活性。我们采用DNA彗星试验来测定（+）-和（-）-松萝酸对小鼠肝脏和肾脏细胞的遗传毒性作用。仅在口服给药1小时后观察到松萝酸的遗传毒性作用。100和50毫克/千克的松萝酸剂量导致肝脏和肾脏细胞中的DNA损伤。松萝酸的遗传毒性作用与细胞中的氧化应激有关。（+）-和（-）-对映体的作用没有显著差异。