白细胞介素-1β的表达会使脊髓损伤后神经系统的预后恶化。
The expression of IL-1β can deteriorate the prognosis of nervous system after spinal cord injury.
作者信息
Li Tao, Li Yu-Tang, Song Di-Yu
机构信息
a Department of Spinal Surgery , Shandong Provincial Hospital Affiliated to Shandong University , Jinan , Shandong , P.R. China.
b Department of Microbiology and Infectious Disease Center , School of Basic Medical Sciences, Peking University Health Science Center , Beijing , P.R. China.
出版信息
Int J Neurosci. 2018 Aug;128(8):778-782. doi: 10.1080/00207454.2018.1424154. Epub 2018 Jan 15.
PURPOSE
We used Anakinra to inhibit the expression of IL-1β based on the model of spinal cord injury in the rat stomach and explored whether it had a certain neuroprotective effect after spinal cord injury.
MATERIALS AND METHODS
The spinal cord injury model of four segments (T5-T8) was prepared by using vascular clamp. Thirty rats were randomized to the control group and the experimental group, and the control group used normal saline, while the experimental group used Anakinra after spinal cord injury. The spinal cord tissue was extracted at 6 h and 24 h after the operation to carry out the histopathological evaluation and to analyze the contents of IL-1β and malondialdehyde and the activities of glutathione peroxidase and superoxide dismutase.
RESULTS
Edema and inflammatory cell infiltration were obviously seen after spinal cord injury, the IL-1β level in serum was significantly increased, but the activity of glutathione peroxidase, superoxide dismutase and catalase was decreased in the control group compared with the experimental group. The experimental group could increase the activity of antioxidant enzymes, but had no significant effect on malondialdehyde.
CONCLUSIONS
Anakinra had a certain protective effect through the inhibition of IL-1β on spinal cord injury.
目的
基于大鼠脊髓损伤模型，使用阿那白滞素抑制白细胞介素-1β（IL-1β）的表达，探讨其在脊髓损伤后是否具有一定的神经保护作用。
材料与方法
采用血管夹制备 T5 - T8 节段的脊髓损伤模型。将 30 只大鼠随机分为对照组和实验组，对照组使用生理盐水，实验组在脊髓损伤后使用阿那白滞素。术后 6 小时和 24 小时提取脊髓组织进行组织病理学评估，并分析 IL-1β、丙二醛的含量以及谷胱甘肽过氧化物酶和超氧化物歧化酶的活性。
结果
脊髓损伤后可见明显的水肿和炎性细胞浸润，对照组血清中 IL-1β水平显著升高，但与实验组相比，对照组谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶的活性降低。实验组可提高抗氧化酶的活性，但对丙二醛无显著影响。
结论
阿那白滞素通过抑制 IL-1β对脊髓损伤具有一定的保护作用。
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