BdCIPK31 的异位表达赋予转基因烟草植株增强的低温耐受性。
Ectopic expression of BdCIPK31 confers enhanced low-temperature tolerance in transgenic tobacco plants.
机构信息
The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China.
出版信息
Acta Biochim Biophys Sin (Shanghai). 2018 Feb 1;50(2):199-208. doi: 10.1093/abbs/gmx140.
Calcineurin B-like protein (CBL), the Ca2+ sensor, and its interacting protein kinases (CIPKs) play essential roles in plants' response to stress. However, few studies have focused on the functions of CIPKs in low-temperature response. In the present study, BdCIPK31, a cold-responsive CIPK in Brachypodium distachyon, was found to participate in low-temperature response. Ectopic expression of BdCIPK31 conferred cold tolerance in transgenic tobaccos. Further analyses indicated that expression of BdCIPK31 improved ROS detoxication and omsoprotectant biosynthesis in transgenic plants under low-temperature treatment, suggesting that the BdCIPK31 functions positively in plant adaption to the cold-induced oxidative and osmotic stresses. Moreover, BdCIPK31 could upregulate the expressions of some representative stress-related genes under cold stress. In conclusion, these findings suggest that BdCIPK31 functions positively in plant cold response.
钙调神经磷酸酶 B 类似蛋白（CBL）是钙离子传感器，其互作蛋白激酶（CIPKs）在植物应对胁迫反应中发挥重要作用。然而，目前关于 CIPKs 在低温响应中的功能的研究较少。本研究在拟南芥中发现冷响应型 CIPK（BdCIPK31）参与低温响应。BdCIPK31 的异位表达赋予了转基因烟草的耐冷性。进一步分析表明，在低温处理下，BdCIPK31 的表达提高了转基因植物中 ROS 解毒和渗透保护剂的生物合成，表明 BdCIPK31 正向调节植物对冷诱导的氧化和渗透胁迫的适应。此外，BdCIPK31 可以在冷胁迫下上调一些代表性的应激相关基因的表达。总之，这些发现表明 BdCIPK31 在植物的冷响应中发挥积极作用。
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