The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, Key Laboratory of Molecular Biophysics MoE, College of Life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan, China.
PLoS One. 2013 Jul 29;8(7):e69881. doi: 10.1371/journal.pone.0069881. Print 2013.
Calcineurin B-like protein-interacting protein kinases (CIPKs) have been found to be responsive to abiotic stress. However, their precise functions and the related molecular mechanisms in abiotic stress tolerance are not completely understood, especially in wheat. In the present study, TaCIPK29 was identified as a new member of CIPK gene family in wheat. TaCIPK29 transcript increased after NaCl, cold, methyl viologen (MV), abscisic acid (ABA) and ethylene treatments. Over-expression of TaCIPK29 in tobacco resulted in increased salt tolerance, which was demonstrated by higher germination rates, longer root lengths and better growth status of transgenic tobacco plants compared to controls when both were treated with salt stress. Physiological measurements indicated that transgenic tobacco seedlings retained high K(+)/Na(+) ratios and Ca(2+) content by up-regulating some transporter genes expression and also possessed lower H2O2 levels and reduced membrane injury by increasing the expression and activities of catalase (CAT) and peroxidase (POD) under salt stress. Moreover, transgenic lines conferred tolerance to oxidative stress by increasing the activity and expression of CAT. Finally, TaCIPK29 was located throughout cells and it preferentially interacted with TaCBL2, TaCBL3, NtCBL2, NtCBL3 and NtCAT1. Taken together, our results showed that TaCIPK29 functions as a positive factor under salt stress and is involved in regulating cations and reactive oxygen species (ROS) homeostasis.
钙调神经磷酸酶 B 样蛋白相互作用蛋白激酶（CIPKs）已被发现对非生物胁迫有反应。然而，它们在非生物胁迫耐受中的精确功能和相关分子机制尚不完全清楚，尤其是在小麦中。在本研究中，TaCIPK29 被鉴定为小麦 CIPK 基因家族的一个新成员。TaCIPK29 的转录水平在 NaCl、冷胁迫、甲基紫精（MV）、脱落酸（ABA）和乙烯处理后增加。在烟草中超表达 TaCIPK29 导致盐胁迫耐受性增强，与对照相比，转 TaCIPK29 烟草在盐胁迫下具有更高的萌发率、更长的根长和更好的生长状态。生理测量表明，转 TaCIPK29 烟草幼苗通过上调一些转运基因的表达，保持了较高的 K+/Na+比值和 Ca2+含量，并且通过增加过氧化氢酶（CAT）和过氧化物酶（POD）的表达和活性，降低了 H2O2 水平和膜损伤。此外，转 TaCIPK29 烟草对氧化胁迫的耐受性通过增加 CAT 的活性和表达来赋予。最后，TaCIPK29 存在于整个细胞中，它优先与 TaCBL2、TaCBL3、NtCBL2、NtCBL3 和 NtCAT1 相互作用。综上所述，我们的研究结果表明，TaCIPK29 在盐胁迫下作为一个正调控因子发挥作用，参与调节阳离子和活性氧（ROS）稳态。
