Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry (Tianjin Normal University), Ministry of Education, Tianjin Key Laboratory of Structure and Performance for Functional Molecule, College of Chemistry, Tianjin Normal University, 393 Binshui West Road, Tianjin 300387, PR China.
Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry (Tianjin Normal University), Ministry of Education, Tianjin Key Laboratory of Structure and Performance for Functional Molecule, College of Chemistry, Tianjin Normal University, 393 Binshui West Road, Tianjin 300387, PR China.
Talanta. 2018 Mar 1;179:478-484. doi: 10.1016/j.talanta.2017.11.042. Epub 2017 Nov 21.
Rare-earth-doped upconversion nanoparticles (UCNPs) behaved advantages such as non-autofluorescence, environment-friendly synthetic route, low cost and simple detection procedures. In addition, the UCNPs show unique upconvering optical property of converting low-energy infrared photons to high-energy visible ones. Here in our work, water and ethylene glycol at the ratio of 1/1 (v/v) were chosen as solvent. Polyethyleneimine (PEI) was chosen as surface additives to synthesize the NaYF:Yb, Tm UCNPs by one-pot route at 200°C. The as-prepared UCNPs exhibited a strong emission at 473nm under the continuous excitation at 980nm. Dopamine (DA), one of the important catecholamine neurotransmitters, shows a crucial role to regulate the memory and attention in brain, renal and hormonal systems. DA molecules could be gently oxidized to quinone structure (ox-DA) in air-containing buffer solution under alkaline condition. In this study, a label-free luminescent probe based on the luminescence energy transfer (LET) between the NaYF:Yb, Tm UCNPs and ox-DA was developed for highly sensitive and selective detection of DA. The luminescent response ratios (I/I) increased linearly with the concentration of DA increasing in the ranges of 0.25 - 150µM. The relative standard deviation (RSD) for 11 replicate detections of 25µM DA was 0.48%, and the limit of detection was 124nM. The developed method was applied for detection of DA in biological fluids with the quantitative spike recoveries from 94.8% to 103.6%.
掺稀土上转换纳米粒子（UCNPs）具有非自发荧光、环保合成路线、低成本和简单检测程序等优点。此外，UCNPs 表现出独特的上转换光学特性，可将低能量的红外光子转换为高能量的可见光。在我们的工作中，选择水和乙二醇的比例为 1/1（v/v）作为溶剂。选择聚乙烯亚胺（PEI）作为表面添加剂，通过一锅法在 200°C 下合成 NaYF:Yb，Tm UCNPs。所制备的 UCNPs 在 980nm 连续激发下于 473nm 处表现出强发射。多巴胺（DA）是一种重要的儿茶酚胺神经递质，在大脑、肾脏和激素系统中对调节记忆和注意力起着至关重要的作用。DA 分子在碱性条件下的含空气缓冲溶液中可被温和氧化为醌结构（ox-DA）。在这项研究中，开发了一种基于 NaYF:Yb，Tm UCNPs 和 ox-DA 之间的荧光能量转移（LET）的无标记荧光探针，用于高灵敏度和选择性检测 DA。荧光响应比（I/I）随 DA 浓度在 0.25-150µM 范围内的增加呈线性增加。25µM DA 的 11 次重复检测的相对标准偏差（RSD）为 0.48%，检测限为 124nM。该方法已应用于生物流体中 DA 的检测，从 94.8%到 103.6%的定量加标回收率。
