Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Talanta. 2018 Mar 1;179:803-809. doi: 10.1016/j.talanta.2017.12.014. Epub 2017 Dec 6.
Novel amperometric bi-enzyme biosensor based on tyrosinase (Tyr)-catalyzed L-tyrosine polymerization to effectively immobilize Tyr and glucose oxidase (GOx) simultaneously has been developed and is demonstrated to be efficient in monitoring multi-analyte (bisphenol A (BPA), phenol, Cr(III), glucose, and Cr(VI)). For the first time, the polymer from L-tyrosine oxidation (PLT) was utilized to in-situ immobilize biomacromolecules and was demonstrated to be effective in entrapping high-load and high-activity enzymes. The prepared bi-enzyme biosensor (PLT-Tyr-GOx/Au electrode) works well in the biosensing of glucose (GOx substrate) and Cr(VI) (GOx inhibitor), and also exhibits excellent performance in Tyr substrates (BPA and phenol) and Tyr inhibitor (Cr(III)) sensing. In addition, the resultant biosensor exhibits excellent stability, precision, high sensitivity and fabrication simplicity, which may find wide applications in diversified fields including biotechnology.
基于酪氨酸酶（Tyr）催化 L-酪氨酸聚合的新型安培双酶生物传感器已被开发出来，该传感器可有效地同时固定 Tyr 和葡萄糖氧化酶（GOx），并被证明可有效监测多分析物（双酚 A（BPA）、苯酚、Cr（III）、葡萄糖和 Cr（VI））。首次利用 L-酪氨酸氧化产物（PLT）原位固定生物大分子，该方法可有效固定高负载量和高活性的酶。所制备的双酶生物传感器（PLT-Tyr-GOx/Au 电极）在葡萄糖（GOx 底物）和 Cr（VI）（GOx 抑制剂）的生物传感中表现良好，并且在 Tyr 底物（BPA 和苯酚）和 Tyr 抑制剂（Cr（III））传感中也表现出优异的性能。此外，所得生物传感器具有出色的稳定性、精度、高灵敏度和制造简单性，这可能使其在生物技术等多个领域得到广泛应用。
