Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Talanta. 2019 Mar 1;194:343-349. doi: 10.1016/j.talanta.2018.10.055. Epub 2018 Oct 18.
In order to realize the multi-analyte assays for physiological molecules and environmental contaminants, a bi-enzyme biosensor based on horseradish peroxidase (HRP)-catalyzed noradrenalin (NA) polymerization in the presence of HO was fabricated for the first time and utilized in immobilizing HRP and glucose oxidase (GOx) simultaneously. The resultant bi-enzyme modified electrode was demonstrated to be efficient in monitoring multi-analyte (HO, Cr(III), glucose, and Cr(VI)). It was shown that the prepared PNA-HRP-GOx/Pt electrode exhibits a sensitivity (S) high up to 628.4 μA mM cm in the linear range (LR) of 0.50 μM ~ 0.42 mM and a S of 208.9 μA mM cm in the LR of 0.42 mM~3.5 mM in glucose sensing, with a limit of detection (LOD) of 0.08 μM observed; in Cr(VI) sensing a LOD of 0.20 nM and a LR of 0.50 ~ 6.0 nM were obtained. With the addition of polyaniline (PANI), the resultant PNA-HRP-GOx/PANI/Pt electrode potentiostated at - 0.20 V responded linearly to HO concentration in 0.05 ~ 30.2 mM range, with linearly responses to Cr(III) concentration in the LR of 0.01 ~ 3.8 μM. Hence, amperometric biosensors with high S, low LOD and good anti-interference ability for detection of glucose, HO and heavy metal ions (Cr(III) and Cr(VI)) were developed.
为了实现对生理分子和环境污染物的多分析物检测，首次制备了基于辣根过氧化物酶（HRP）在 HO 存在下催化去甲肾上腺素（NA）聚合的双酶生物传感器，并用于同时固定 HRP 和葡萄糖氧化酶（GOx）。结果表明，这种双酶修饰电极能够有效地监测多分析物（HO、Cr(III)、葡萄糖和 Cr(VI)）。所制备的 PNA-HRP-GOx/Pt 电极在 0.50 μM0.42 mM 的线性范围内具有高达 628.4 μA mM cm 的灵敏度（S），在葡萄糖检测的线性范围内具有 208.9 μA mM cm 的灵敏度（S），在 0.42 mM3.5 mM 范围内，检测限（LOD）为 0.08 μM；在 Cr(VI)检测中，检测限（LOD）为 0.20 nM，线性范围为 0.506.0 nM。通过添加聚苯胺（PANI），所得的 PNA-HRP-GOx/PANI/Pt 电极在 -0.20 V 下电测定对 0.0530.2 mM 范围内的 HO 浓度呈线性响应，对 0.01~3.8 μM 范围内的 Cr(III)浓度呈线性响应。因此，开发了具有高灵敏度、低检测限和良好抗干扰能力的安培生物传感器，用于检测葡萄糖、HO 和重金属离子（Cr(III)和 Cr(VI)）。
