Christen Jacques-Robert, Edmond Edwin, Drancourt Michel
URMITE, UMR CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée Infection, Aix Marseille Université, 19-21 Bd Jean Moulin, 13005, Marseille, France.
Department of Infectious Diseases and Tropical Medicine, Laveran Military Teaching Hospital, Marseille, France.
BMC Res Notes. 2018 Jan 8;11(1):11. doi: 10.1186/s13104-017-3119-2.
Gemmata bacteria are fastidious, Gram-negative and aerobic. The only representatives are Gemmata obscuriglobus and Gemmata massiliana. These Planctomycetes appear to be a part of human digestive tract microbiome, and G. massiliana has been isolated from water. Further specific detection in the blood of two patients with febrile neutropenia suggests that Gemmata bacteremia may remain under-documented. The objective of this study was to develop an effective protocol to document Gemmata spp. bacteremia in the laboratory. Using mock-infected and control blood specimens, three methods for detecting Gemmata bacteremia, namely, automated microbial detection, culture on solid medium, and quantitative polymerase chain reaction (PCR), have been developed and studied.
Gemmata spp. were undetected by automated blood culture system but culturing mock-infected blood on Caulobacter agar detected ≥ 10 G. obscuriglobus bacteria/mL and ≥ 10 G. massiliana bacteria/mL. Specific real-time PCR detected 10 Gemmata bacteria/mL. These protocols may be used to investigate the epidemiology of Gemmata spp. bacteremia.
芽殖菌属细菌苛求、革兰氏阴性且需氧。仅有的代表菌种是隐匿芽殖菌和马赛芽殖菌。这些浮霉菌门细菌似乎是人类消化道微生物群的一部分，并且马赛芽殖菌已从水中分离出来。对两名发热性中性粒细胞减少症患者血液的进一步特异性检测表明，芽殖菌属菌血症可能仍记录不足。本研究的目的是制定一种在实验室记录芽殖菌属菌血症的有效方案。使用模拟感染和对照血液标本，已开发并研究了三种检测芽殖菌属菌血症的方法，即自动微生物检测、固体培养基培养和定量聚合酶链反应（PCR）。
自动血培养系统未检测到芽殖菌属细菌，但在柄杆菌琼脂上培养模拟感染血液可检测到每毫升≥10株隐匿芽殖菌和每毫升≥10株马赛芽殖菌。特异性实时PCR检测到每毫升10株芽殖菌。这些方案可用于调查芽殖菌属菌血症的流行病学。
