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马赛吉玛塔菌新种(Gemmata massiliana sp. nov)的基因组草图,这是一种呈现两种变体的水生浮霉菌门物种。

Draft genome of Gemmata massiliana sp. nov, a water-borne Planctomycetes species exhibiting two variants.

作者信息

Aghnatios Rita, Cayrou Caroline, Garibal Marc, Robert Catherine, Azza Said, Raoult Didier, Drancourt Michel

机构信息

Aix-Marseille Université, URMITE, UM63, CNRS7278, IRD198, Inserm 1095, Faculté de médecine, 27 Boulevard jean Moulin, 13385 Marseille, cedex 05, France.

出版信息

Stand Genomic Sci. 2015 Dec 7;10:120. doi: 10.1186/s40793-015-0103-0. eCollection 2015.

Abstract

Gemmata massiliana is a new Planctomycetes bacterium isolated from a hospital water network in France, using a new culture medium. It is an aerobic microorganism with optimal growth at pH 8, at 30 °C and salinity ≤ 1.25 % NaCl. G. massiliana is resistant to β-lactam antibiotics, due to lack of peptidoglycan in its cell wall.G. massiliana shares a 97 % 16S rRNA gene sequence similarity with the nearest species, Gemmata obscuriglobus; and 99 % similarity with unnamed soil isolates. Its 9,249,437-bp genome consists in one chromosome and no detectable plasmid and has a 64.07 % G + C content, 32.94 % of genes encoding for hypothetical proteins. The genome contains an incomplete 19.6-kb phage sequence, 26 CRISPRs, 3 CAS and 15 clusters of secondary metabolites. G. massiliana genome increases knowledge of a poorly known world of bacteria.

摘要

马赛芽球菌是一种从法国医院供水网络中分离出来的新型浮霉菌门细菌,使用的是一种新型培养基。它是一种需氧微生物,在pH值为8、温度为30°C且盐度≤1.25%氯化钠的条件下生长最佳。由于其细胞壁中缺乏肽聚糖,马赛芽球菌对β-内酰胺类抗生素具有抗性。马赛芽球菌与最接近的物种隐秘芽球菌的16S rRNA基因序列相似度为97%;与未命名的土壤分离株相似度为99%。其9249437碱基对的基因组由一条染色体组成,未检测到质粒,G+C含量为64.07%,32.94%的基因编码假设蛋白。该基因组包含一个不完整的19.6kb噬菌体序列、26个成簇规律间隔短回文重复序列(CRISPRs)、3个CRISPR相关蛋白(CAS)和15个次生代谢产物簇。马赛芽球菌基因组增加了对一个鲜为人知的细菌世界的了解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b08c/4672568/5c3f4685b297/40793_2015_103_Fig1_HTML.jpg

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