Ibeas Miguel A, Grant-Grant Susana, Navarro Nathalia, Perez M F, Roschzttardtz Hannetz
Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile, Santiago, Chile.
Departamento de Ecología, Pontificia Universidad Católica de Chile, Santiago, Chile.
Front Plant Sci. 2017 Dec 22;8:2186. doi: 10.3389/fpls.2017.02186. eCollection 2017.
Iron is an essential micronutrient for plants. Little is know about how iron is loaded in embryo during seed development. In this article we used Perls/DAB staining in order to reveal iron localization at the cellular and subcellular levels in different Brassicaceae seed species. In dry seeds of , and iron localizes in vacuoles of cells surrounding provasculature in cotyledons and hypocotyl. Using and as model plants we determined where iron localizes during seed development. Our results indicate that iron is not detectable by Perls/DAB staining in heart stage embryo cells. Interestingly, at torpedo development stage iron localizes in nuclei of different cells type, including integument, free cell endosperm and almost all embryo cells. Later, iron is detected in cytoplasmic structures in different embryo cell types. Our results indicate that iron accumulates in nuclei in specific stages of embryo maturation before to be localized in vacuoles of cells surrounding provasculature in mature seeds.
铁是植物必需的微量营养素。关于种子发育过程中铁如何在胚中积累，人们了解甚少。在本文中，我们使用佩尔斯/二氨基联苯胺（Perls/DAB）染色法，以揭示不同十字花科种子物种在细胞和亚细胞水平上的铁定位。在[具体植物A]、[具体植物B]和[具体植物C]的干燥种子中，铁定位于子叶和下胚轴中围绕原维管束的细胞液泡中。以[具体植物D]和[具体植物E]作为模式植物，我们确定了种子发育过程中铁的定位位置。我们的结果表明，在心形期胚细胞中，佩尔斯/二氨基联苯胺染色法无法检测到铁。有趣的是，在鱼雷形发育阶段，铁定位于不同细胞类型的细胞核中，包括珠被、游离细胞胚乳和几乎所有胚细胞。后来，在不同胚细胞类型的细胞质结构中检测到铁。我们的结果表明，铁在胚成熟的特定阶段积累于细胞核中，然后才定位于成熟种子中围绕原维管束的细胞液泡中。
