Rosenthal Matthew, Huang Bin, Katabi Nora, Migliacci Jocelyn, Bryant Robert, Kaplan Samuel, Blackwell Timothy, Patel Snehal, Yang Liying, Pei Zhiheng, Tang Yi-Wei, Ganly Ian
Head and Neck Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Clinical Microbiology Service, Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Oncotarget. 2017 Nov 25;8(65):109393-109401. doi: 10.18632/oncotarget.22682. eCollection 2017 Dec 12.
The majority of patients diagnosed with oropharyngeal squamous cell cancer (OPSCC) are due to HPV infection. At present, there are no reliable tests for screening HPV in patients with OPSCC. The objective of this study was to assess the Cobas® HPV Test on oral rinse specimens as an early, non-invasive tool for HPV-related OPSCC.
Oral rinse specimens were collected from 187 patients (45 with OPSCC, 61 with oral cavity SCC (OCSCC) and 81 control patients who had benign or malignant thyroid nodules) treated at MSKCC. The Cobas® HPV Test was used to detect 14 high-risk HPV types in these samples. Performance of the HPV Test was correlated with p16 tumor immunohistochemistry as gold standard.
91.1% of the oropharynx cancer patients had p16 positive tumors compared to 3.3% of oral cavity cancer. Of the 81 control patients, 79 (97.5%) had no HPV in their oral rinse giving a specificity of the HPV test of 98%. For the combined oral cavity oropharynx cancer cohort, the sensitivity, specificity, positive predictive value and negative predictive value of the HPV Test were 79.1%, 90.5%, 85.0% and 86.4% respectively when p16 immunohistochemistry was used as the reference.
The Cobas® HPV Test on oral rinse is a highly specific and potentially sensitive test for oropharyngeal cancer and may be a potentially useful screening test for early oropharyngeal cancer.
We describe an oral rinse test for the detection of HPV related oropharyngeal cancer.
大多数被诊断为口咽鳞状细胞癌（OPSCC）的患者是由人乳头瘤病毒（HPV）感染引起的。目前，尚无可靠的检测方法用于筛查OPSCC患者中的HPV。本研究的目的是评估Cobas® HPV检测在口腔冲洗样本中作为HPV相关OPSCC的早期非侵入性工具的效果。
从在纪念斯隆凯特琳癌症中心（MSKCC）接受治疗的187例患者中收集口腔冲洗样本，其中45例患有OPSCC，61例患有口腔鳞状细胞癌（OCSCC），81例为患有良性或恶性甲状腺结节的对照患者。使用Cobas® HPV检测来检测这些样本中的14种高危HPV类型。HPV检测的性能与作为金标准的p16肿瘤免疫组织化学相关联。
91.1%的口咽癌患者肿瘤p16呈阳性，而口腔癌患者中这一比例为3.3%。在81例对照患者中，79例（97.5%）口腔冲洗样本中未检测到HPV，HPV检测的特异性为98%。对于口腔癌和口咽癌联合队列，以p16免疫组织化学作为参考时，HPV检测的敏感性、特异性、阳性预测值和阴性预测值分别为79.1%、90.5%、85.0%和86.4%。
口腔冲洗样本的Cobas® HPV检测对口咽癌具有高度特异性且可能具有敏感性，可能是早期口咽癌的一种潜在有用的筛查检测方法。
我们描述了一种用于检测HPV相关口咽癌的口腔冲洗检测方法。
