State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, People's Republic of China.
School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, People's Republic of China.
Bioprocess Biosyst Eng. 2018 Apr;41(4):489-499. doi: 10.1007/s00449-017-1883-y. Epub 2018 Jan 8.
The lycopene biosynthetic genes crtE, crtB, and crtI from Deinococcus wulumuqiensis R12 were integrated into three different vector backbones-pET28a, pTrc99A, and pUC18-and the resulting recombinant plasmids pET28a-EBI, pTrc99A-EBI, and pUC18-EBI were introduced into different Escherichia coli hosts. The results showed that lycopene production of strain 28BL was lower than that of 99 series strains without IPTG in LB medium. In addition, lycopene production of 99JM with supplementation of 20% (w/v) glycerol was 1.6-fold higher than with supplementation of 6% (w/v) glucose. After optimization of the host strain and culture medium, the yield of microbial lycopene was increased successfully. When recombinant E. coli 99DH was cultivated under exposure to light in 2YT + Gly medium, the highest lycopene production rate was 26.2 mg/L/h at 30 h, and the maximum specific lycopene content was 67 mg/g dry cell (925 mg/L) at 40 h, which represents a 76% increase over the starting point.
来自极端嗜热古菌沃尔穆轮球菌（Deinococcus wulumuqiensis R12）的番茄红素生物合成基因 crtE、crtB 和 crtI 被整合到三个不同的载体骨架中——pET28a、pTrc99A 和 pUC18——并将所得重组质粒 pET28a-EBI、pTrc99A-EBI 和 pUC18-EBI 导入不同的大肠杆菌宿主中。结果表明，在 LB 培养基中，没有 IPTG 时，28BL 菌株的番茄红素产量低于 99 系列菌株。此外，在补充 20%（w/v）甘油的情况下，99JM 的番茄红素产量比补充 6%（w/v）葡萄糖的产量高 1.6 倍。通过优化宿主菌株和培养基，成功提高了微生物番茄红素的产量。当重组大肠杆菌 99DH 在 2YT+Gly 培养基中光照条件下培养时，在 30 h 时达到最高的番茄红素生产速率 26.2 mg/L/h，在 40 h 时达到最高的比番茄红素含量 67 mg/g 干细胞（925 mg/L），与起始点相比提高了 76%。
