皮下注射CpG-寡脱氧核苷酸可作为一种有效的佐剂，用于一种基于HIV-1反式激活因子的候选疫苗，以在BALB/c小鼠中引发细胞免疫。

Subcutaneous administration CpG-ODNs acts as a potent adjuvant for an HIV-1-tat-based vaccine candidate to elicit cellular immunity in BALB/c mice.

作者信息

Panahi Zeinab, Abdoli Asghar, Mosayebi Ghasem, Mahdavi Mehdi, Bahrami Fariborz

机构信息

Department of Microbiology and Immunology, School of Medicine, Molecular and Medicine Research Center (MMRC), Arak University of Medical Sciences, P.O. Box: 38481-7-6941, Arak, Iran.

Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.

出版信息

Biotechnol Lett. 2018 Mar;40(3):527-533. doi: 10.1007/s10529-017-2497-9. Epub 2018 Jan 8.

DOI:10.1007/s10529-017-2497-9

PMID:29313255

Abstract

OBJECTIVE

To evaluate the combined effects of CpG oligodeoxynucleotides (CpG-ODNs) adjuvant and subcutaneous injection route on efficacy of a HIV-1-tat DNA vaccine candidate using BALB/c mice as an animal model.

RESULTS

Evaluation of cellular and humoral immunity of mice injected subcutaneously with HIV-1-tat gene cloned into a pcDNA3.1 vector indicated that significant levels of IFN-γ cytokine secretion (900 pg/ml), lymphocyte proliferation (2.5 stimulation index) and IgG (1.45 absorbance 450 nm) production could be achieved. These indicators of stimulated cellular immunity were elicited 2 weeks after the last injection (P < 0.05).

CONCLUSIONS

Formulation of HIV-1-tat DNA vaccine candidate with CpG-ODNs as an adjuvant while administrated subcutaneously are a promising approach to induce effective cellular immunity responses against HIV-1 infection.

摘要

目的

以BALB/c小鼠为动物模型，评估CpG寡脱氧核苷酸（CpG-ODNs）佐剂和皮下注射途径对一种HIV-1-tat DNA候选疫苗效力的联合作用。

结果

对皮下注射克隆于pcDNA3.1载体的HIV-1-tat基因的小鼠的细胞免疫和体液免疫进行评估，结果表明可实现显著水平的IFN-γ细胞因子分泌（900 pg/ml）、淋巴细胞增殖（2.5刺激指数）和IgG产生（450 nm处吸光度为1.45）。这些刺激细胞免疫的指标在最后一次注射后2周出现（P < 0.05）。